| 摘要: |
| 摘要：【目的】探究红地球葡萄延迟栽培模式下，增加土壤水分对转色期葡萄果肉代谢组特征的影响。【方法】以延迟栽培的红地球葡萄为试材，在葡萄转色前20天左右，利用自动灌溉系统控制土壤体积含水率分别达到0.15 m3/m3（低水分）、0.18 m3/m3（中水分）和0.24 m3/m3（高水分），并维持到试验结束。当低水分处理果实进入转色时采样，并采用液质联用（LC-MS）技术分析比较不同水分处理下果肉非靶向代谢组学差异。【结果】随着土壤供水量降低，果实可溶性固形物量增加。代谢组数据显示，与高水分处理相比，低水分处理的果肉中出现27个差异代谢物（POS），其中鸢尾苷、乙酰丙酸等11种代谢物的FC值（差异倍数，Fold Change）上调，丁醛类等16种代谢物物FC值下调，是果实成熟度高的特征；中水分和高水分处理相比，葡萄果肉有13种差异代谢物上调，20种下调，其中甲酮和白藜芦醇二聚体（下调）的FC值较小；与高水分相比，低水分和中水分处理富集到的代谢途径中有8条相同，且代谢途径中主要的差异代谢物均指向丙酮酸。【结论】高的土壤含水率明显延缓果实的成熟进程，包括可溶性固形物降低，与色素积累、香气、细胞壁结构等相关的代谢产物明显降低；丙酮酸是葡萄转色期最活跃的代谢物。 |
| 关键词: 萄；延迟栽培；转色期；水分；代谢组 |
| DOI： |
| 分类号:S628 |
| 基金项目:国家自然科学基金地区基金项目（31660585）；国家现代农业产业技术体系兰州葡萄综合试验站（CARS-29-23）；葡萄种质创新与提质增效关键技术研究与示范(18ZD2NA006-4) |
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| Response of Metabolic Group Change in Grape Pulp to Soil Moisture at Veraison Stage |
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zhang kun
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WEI FANG UNIVERSITY
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| Abstract: |
| Abstract:【Background】The delayed cultivation of grape is mainly concentrated in northwest and Northeast China,where the accumulated temperature can not meet the normal ripening of grape,the grape sales time could be delayed to December each year,and with high economic benefits.The maturity regulation is an important aspect of grape delayed cultivation.Previous studies focused on the regulation of light and temperature,but few on the regulation of water during grape ripening period,and water regulation is the most easily realized agronomic measure in actual production.【Objective】To explore the effect of increasing soil moisture on the metabolism of‘Red Globe’grape pulp under the delayed cultivation.【Method】This test took the‘Red Globe’grape of delayed cultivation as the material,and about 20 days before veraison,the soil volume moisture content controlled by the automatic irrigation system were 0.15 m3·m-3（low moisture）,0.18 m3·m-3（medium moisture）and 0.24 m3·m-3(high moisture),respectively,and maintained until the end of the experiment.The non-target metabonomics of pulp under different water treatments were analyzed and compared by LC-MS.【Result】With the decrease of soil water supply,the accumulation of soluble solids in grape fruits were increased in the same period.The metabolomics showed that there were 27 different metabolites(POS)in pulp of low moisture treatment compared with high moisture treatment,among them,the fold change values(FC)of 11 metabolites such as tectoridin and levulinic acid were up-regulated,while 16 metabolites such as butyraldehyde were down regulated,which were the characteristics of high fruit maturity.There were 13 up-regulated and 21 down-regulated metabolites in medium moisture treatment compared with high moisture treatment,and the FC value of methanone and epsilon-viniferin(down-regulated)were relatively small.There were 8 same metabolic pathways in the enriched metabolic pathway for low moisture treatment and medium moisture treatment compared with high moisture treatment,and the main metabolites in the metabolic pathway was pyruvate.【Conclusion】The high soil water content significantly delayed the ripening process of grape fruit,including the decrease of soluble solids,and the decrease of metabolites related to pigment accumulation,aroma and cell wall structure and so on.Pyruvate may be the most active metabolite at?veraison?stage. |
| Key words: grape;delayed cultivation;veraison;water ;metabolomic |
