| 引用本文: | 李松旌,樊向阳,崔二苹,等.滴头流量对再生水灌溉作物根区典型微生物群落结构的影响[J].灌溉排水学报,0,():-. |
| lisongjing,fanxiangyang,cuierping,et al.滴头流量对再生水灌溉作物根区典型微生物群落结构的影响[J].灌溉排水学报,0,():-. |
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| 滴头流量对再生水灌溉作物根区典型微生物群落结构的影响 |
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李松旌1,2, 樊向阳1,3,4, 崔二苹1,3,4, 李胜曙1,3,4, 胡超1,3,4, 崔丙健1,3,4, 刘源1,3,4, 李中阳1,3,4, 赵志娟1,3,4
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1.中国农业科学院农田灌溉研究所;2.中国农业科学院研究生院;3.中国农业科学院农业水资源高效安全利用重点开放实验室;4.中国农业科学院新乡农业水土环境野外科学观测试验站
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| 摘要: |
| 【目的】探明再生水滴灌条件下不同滴头流量对根区土壤典型微生物群落结构及功能的影响和差异性。【方法】采用盆栽试验，以传统漫灌为对照，研究了滴灌滴头流量（2、4、8 L/h）下番茄根区不同土层深度微生物群落分布规律的差异性及其影响因素。【结果】滴灌处理微生物多样性指数均高于对照组漫灌处理，且在5～10 cm土层的差异达到了显著水平（P<0.05）；不同滴头流量处理对番茄根区门水平、属水平优势菌群并无影响，但可通过影响根区土壤pH、EC及全磷质量分数等环境因子进而影响微生物群落的相对丰度，如滴头流量2 L/h处理较其他处理增加了10～15 cm土层中全磷质量分数，进而显著增加了拟杆菌门（Bacteroidetes）相对丰度，滴灌各处理增加了0～15 cm土层pH值进而降低了鞘脂单胞菌属（Sphingomonas）的相对丰度；滴头流量为2 L/h时0～5 cm土层各功能微生物通路丰度最高。【结论】再生水滴灌条件下不同滴头流量可通过改变土壤理化性状，进而改变作物根区土壤微生物群落结构及分布特征，并提高部分功能菌群丰度，说明适宜滴头流量的选择对于再生水灌溉土壤微生物调控具有重要意义。 |
| 关键词: 再生水;滴灌;滴头流量;根区土壤;微生物群落 |
| DOI： |
| 分类号:X172 |
| 基金项目:国家自然科学基金项目（51479201）；中央级公益性科研院所基本科研业务费专项（FIRI202003-01）；河南省科技攻关项目（192102110094） |
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| Effects of Drippers Flow Rates on Typical Microbial Community Structure in Root Zone Soil of Crops Irrigated with Reclaimed Water |
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lisongjing1,2, fanxiangyang1,3,4, cuierping1,3,4, lishengshu1,3,4, huchao1,3,4, cuibingjian1,3,4, liuyuan1,3,4, lizhongyang1,3,4, zhaozhijuan1,3,4
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1.Farmland Irrigation Research Institute, Chinese Academy of Agricultural Sciences;2.Graduate School of Chinese Academy of Agricultural Sciences;3.Key Laboratory of High-efficient and Safe Utilization of Agriculture Water Resources, Chinese Academy of Agricultural Sciences;4.Agricultural Water Soil Environmental Field Research Station of Xinxiang
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| Abstract: |
| As an important part of the soil ecosystem, soil microorganisms play an important role in the formation of soil structure, the circulation of nutrients, the adsorption and degradation of pollutants. Root zone soil is an active interface for material exchange in the soil-plant system, and its nutrient, enzyme activity and microbial community structure directly determine the growth of plants. Different irrigation methods will cause differences in the distribution of soil moisture and soil physical and chemical properties, which may lead to differences in soil microbial structure. However, reclaimed water as a potential source of agricultural irrigation, there are few reports on the structure and function of soil microbial community from the perspective of irrigation rate for different dripper flow rates with reclaimed water irrigation. 【Objective】In order to explore the effects and differences of emitter flow rates on the structure and function of soil typical microbial community in the root zone soil under drip irrigation with reclaimed water. 【Method】The experiment was conducted in the intelligent artificial climate chamber of Xinxiang Agricultural Soil and Water Environment Field Scientific Observation and Experimental Station, Chinese Academy of Agricultural Sciences from November 2019 to January 2020. The pot experiment was carried out to study the effect of microbial community distribution and its influencing factors at different tomato root zone soil layers under drip irrigation (2, 4, 8 L/h) with traditional flood irrigation as the control. After the experiment, microbial community structure indexes of soil samples were determined by 16S rDNA microbial diversity sequencing method.【Result】The study found that the microbial diversity index of each drip irrigation treatment was higher than that of the control flood irrigation treatment, and the difference reached a significant level in the 5～10 cm soil layer (P<0.05). Different treatments had no effect on the dominant flora at the phylum level and genus level, but the relative abundance of the microbial community could be affected by affecting the soil environmental factors in the tomato root zone (such as pH, EC, total phosphorus, etc.). The emitter flow rate of 2 L/h treatment increased the total phosphorus content in the soil layer of 10～15 cm compared with other treatments, thereby significantly increased the relative abundance of Bacteroidetes. Compared with the control group, the drip irrigation treatments increased the pH of soil layer from 10～15 cm, and reduced the relative abundance of Sphingomonas. When the flow rate of the emitter was 2 L/h and in the 0～5 cm soil layer, the abundance of each functional microbial pathway was the highest. 【Conclusion】The results showed that different emitter flow rates under reclaimed water irrigation can change the physical and chemical properties of the soil, thereby changing the soil microbial community structure and distribution characteristics in the root zone of crops, and increasing the abundance of some functional flora. This study indicated that the selection of appropriate drip irrigation rate is of great significance to the regulation of soil microorganisms in reclaimed water irrigation. |
| Key words: reclaimed water irrigation; drip irrigation; flow rates of dripper; root zone soil; microflora |
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