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引用本文:高彦婷,杨昌钰,张  芮,等.水分胁迫对温室滴灌葡萄根际土壤微生物碳源代谢活性的影响[J].灌溉排水学报,0,():-.
gaoyanting,yangchangyu,zhangrui,et al.水分胁迫对温室滴灌葡萄根际土壤微生物碳源代谢活性的影响[J].灌溉排水学报,0,():-.
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水分胁迫对温室滴灌葡萄根际土壤微生物碳源代谢活性的影响
高彦婷1, 杨昌钰1, 张  芮1, 蔺宝军2, 董博3, 李红霞1
1.甘肃农业大学水利水电工程学院;2.大禹节水集团股份有限公司;3.甘肃农业科学院
摘要:
【目的】探究干旱半干旱地区温室葡萄根际土壤微生物碳源代谢对不同滴灌水平的响应机制。【方法】采用单因素完全随机试验,运用Biolog技术分析了土壤微生物碳源代谢活性在不同水分处理水平(中度胁迫W1处理、轻度胁迫W2处理、充分供水对照CK)下的变化特征。【结果】设施葡萄根际土壤中单糖\糖苷\聚合糖类、氨基酸类、醇类、酸类等碳源利用程度较高,代表碳源有葡萄糖-1-磷酸盐、D-纤维二糖、甘氨酰-L-谷氨酸、I-赤藻糖醇、4-羟基苯甲酸、r-羟基丁酸;2种胁迫水平均能提升土壤微生物碳源代谢活性,胁迫初期W2处理土壤微生物碳源利用能力最高,中、后期W1处理碳源利用能力最高;轻度胁迫条件下总产和单果均质量分别提高11.95%、2.62%,中度胁迫则减产。【结论】水分胁迫可以提高温室葡萄根际土壤微生物活性,强化其总体碳源代谢强度,胁迫初期W2处理代谢最强,中、后期W1处理最强。
关键词:  滴灌;温室葡萄;土壤微生态环境;水分胁迫
DOI:
分类号:S663.1;S628
基金项目:国家自然科学基金(51569002);陇原青年创新创业人才项目(2019-9);甘肃农业大学水利水电工程学院青年教师科技创新(SLXY-QN2021-01);甘肃省教育厅2021年度高等学校创新基金项目(2021B -139);甘肃农业大学青年导师基金项目(GSAU-QDFC-2018-06)
Effects of Water Stress on Microbial Carbon Metabolism in Rhizosphere Soil of Greenhouse Grape
gaoyanting1, yangchangyu1, zhangrui1, linbaojun2, dongbo3, lihongxia1
1.Gansu Agricultural University;2.Da Yu water saving group Limited by Share Ltd,Gansu province;3.Academy of Agricultural Sciences,Gansu province
Abstract:
Abstract:【Objective】To explore the response mechanism of soil microbial carbon source metabolism in the greenhouse grape rhizosphere to different drip irrigation levels in arid and semi-arid regions.【Method】A single factor completely randomized experiment was used to analyze the change characteristics of soil microbial carbon source metabolic activity under different water treatment levels (moderate stress W1 treatment, mild stress W2 treatment, and adequate water supply control CK).【Result】The utilization of monosaccharides, glycosides, polymerized sugars, amino acids, alcohols, acids and other carbon sources in the rhizosphere soil of greenhouse grapes is relatively high. The representative carbon sources are glucose-1-phosphate and D-cellobiose, Glycyl-L-glutamic acid,I-erythritol,4-hydroxybenzoic acid,r-hydroxybutyric acid; two stress levels can increase the metabolic activity of soil microbial carbon sources, and W2 treatment of soil microbes at the initial stage of stress. The carbon source utilization capacity was the highest, and the soil microbial carbon source utilization capacity was the highest in the middle and late stage W1 treatment; the total yield and the average weight of single fruit increased by 11.95% and 2.62% under mild stress conditions, and reduced yield under moderate stress.【Conclusion】Water stress can increase the soil microbial activity in the rhizosphere of greenhouse grapes and strengthen the overall carbon source metabolism. The W2 treatment has the strongest metabolic intensity at the initial stage of the stress, and the W1 treatment at the middle and late stages of the stress.
Key words:  Drip irrigation;Greenhouse grapes;Soil micro-ecological environment;Water stress