| 引用本文: | 张云鹤,高大鹏,王晓蕾,等.盐碱胁迫对水稻苗期光系统Ⅱ性能的影响[J].灌溉排水学报,2022,41(9):52-60. |
| ZHANG Yunhe,GAO Dapeng,WANG Xiaolei,et al.盐碱胁迫对水稻苗期光系统Ⅱ性能的影响[J].灌溉排水学报,2022,41(9):52-60. |
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| 摘要: |
| 【目的】阐明盐碱胁迫下水稻幼苗的光合生理响应机制。【方法】以长白9号和白粳1号为供试材料，设置0、40、80和120 mmol/L共4个物质的量浓度混合盐碱（NaCl∶Na2SO4∶Na2CO3∶NaHCO3=1∶9∶1∶9）处理，通过JIP-test分析快速叶绿素荧光诱导动力学曲线，探究混合盐碱胁迫下水稻幼苗叶片光系统II的变化。【结果】盐碱物质的量浓度在0~80 mmol/L时，2个品种水稻幼苗的叶绿素荧光动力学曲线为典型的OJIP曲线，相同时间点各处理的荧光强度从J点开始出现明显差异。盐碱物质的量浓度升高到120 mmol/L时，2个品种的OJIP曲线从J点开始到P点逐渐趋于平缓，并且与CK相比I点和P点荧光强度显著下降。长白9号和白粳1号的盐碱物质的量浓度与性能指数PIABS、量子产额（φEo、φRo、ψEo）、供体侧性能指数（WK）和受体侧性能指数（VJ）之间存在极显著的非线性关系。随着盐碱物质的量浓度的增加，长白9号和白粳1号的水稻幼苗的地上部含水率、叶片叶绿素a量、叶片叶绿素b量、地上部干物质、最大光化学效率（FV/FM）、量子产额与效率（φEo、φRo、ψEo）和性能指数PIABS等参数逐渐减小，同时供体侧性能指数（WK）、受体侧性能指数（VJ）和热耗散比率（φDo）逐渐递增。2个水稻品种的地上部含水率、叶片叶绿素量以及叶绿素荧光参数随着盐碱物质的量浓度的升高，变化幅度逐渐增大。【结论】盐碱胁迫严重损伤供/受体侧性能，尤其是受体侧性能VJ，使水稻幼苗PSII的电子传输受阻，量子产额下降，降低光系统Ⅱ性能，水稻叶片发生光抑制，降低叶片的光能利用率，水稻幼苗干物质积累受到抑制，导致水稻幼苗的生长发育受到抑制。且盐碱物质的量浓度与PSII性能指数PIABS、VJ、WK以及量子产额之间存在极显著的非线性关系。 |
| 关键词: 水稻；盐碱混合胁迫；光系统Ⅱ |
| DOI：10.13522/j.cnki.ggps.2022002 |
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| Effects of Soil Salinity on Photosystem II of Rice Seedlings |
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ZHANG Yunhe, GAO Dapeng, WANG Xiaolei, SHAO Xiwen, GUO Liying, HUANG Jinrui, GENG Yanqiu
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Jilin Agricultural University, Changchun 130118, China
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| Abstract: |
| 【Objective】 Photosystem II (water-plastoquinone oxidoreductase) is a protein complex in light-dependent reactions of oxygenic photosynthesis. The purpose of this paper is to study the effect of salt stress on photosystem II of rice seedlings. 【Method】We used the varieties of Changbai 9 and Baijing 1 as the model plants. The salt was a mixture of salts at ratios of NaCl∶Na2SO4∶Na2CO3∶NaHCO3=1∶9∶1∶9. Solution of this salt mixture at concentrations from 0 to 120 mmol/L was added to the soil with the irrigation water. In each treatment, we measured and calculated the rapid chlorophyll fluorescence induction kinetic curve using the JIP-test, from which we calculated the the change in photosystem II in the leaves of the rice seedlings.【Result】When salt concentration was in 0~80 mmol/L, the chlorophyll fluorescence kinetic curve of the rice seedlings of both varieties was a typical OJIP curve, and the fluorescence intensity in each treatments differed significantly from the Point J. When salt concentration was increased to 120 mmol/L, the OJIP curve of the two varieties flattened, changing from the Point J to Point P, with the fluorescence intensity at Point I and Point P decreasing remarkably compared to CK (without salinity). There were non-linear relationships between salt concentration and the index PIABS, (φEo, φRo, ψEo), donor-side index (WK) and the recipient-side index (VJ) for both varieties. With the increase in soil salinity, water content, the contents of chlorophyll a and b, maximum photochemical efficiency (FV/FM), quantum yield and efficiency (φEo, φRo, ψEo), the index PIABS of both varieties all gradually decreased, while WK, VJ, and the heat dissipation ratio (φDo) gradually increased. The change in water content, chlorophyll content and chlorophyll fluorescence parameters of the two varieties gradually increased with the increase in salt concentration. 【Conclusion】Saline-alkali stress severely damages the donor/acceptor laterality, especially the acceptor lateral performance index VJ, hindering electron transmission and reducing the quantum yield and the performance of photosystem II. PSII performance index PIABS, VJ, WK and quantum yield are nonlinearly correlated with the salt concentration. |
| Key words: rice; salt-alkali mixed stress; optical system II |
