| 引用本文: | 王帅兵,孟会生,武 欣,等.不同形态氮肥配施固氮菌对复垦土壤酶活性及氮素形态的影响[J].灌溉排水学报,2022,41(11):45-49. |
| WANG Shuaibing,MENG Huisheng,WU Xin,et al.不同形态氮肥配施固氮菌对复垦土壤酶活性及氮素形态的影响[J].灌溉排水学报,2022,41(11):45-49. |
|
| 摘要: |
| 【目的】探究不同形态氮肥配施固氮菌对复垦土壤酶活性及氮素形态的影响。【方法】选取采煤塌陷区复垦1 a的土壤为研究对象，通过盆栽试验分析各形态氮肥与固氮菌配施对土壤微生物量碳、微生物量氮、酶活性、全氮、溶解性总氮、铵态氮、硝态氮的影响。【结果】硝铵态氮肥配施固氮菌可有效提高复垦土壤微生物量碳、微生物量氮、溶解性总氮、铵态氮量，与硝铵态氮肥处理相比，土壤过氧化氢酶、蔗糖酶、蛋白酶、脲酶活性分别提高了4.96%、17.85%、12.53%、6.12%。土壤养分、酶活性、微生物量碳、微生物量氮之间的关系密切，蔗糖酶、蛋白酶、脲酶活性与土壤全氮、溶解性总氮、铵态氮量均呈极显著正相关（P<0.01），与硝态氮量呈显著正相关（P<0.05），土壤微生物量碳、微生物量氮均与溶解性总氮量呈显著正相关（P<0.05）。主成分分析表明，硝铵态氮肥与固氮菌配施处理下的复垦土壤质量最高。【结论】硝铵态氮肥配施固氮菌最有利于提高复垦土壤的微生物活性与养分水平，是一种合理的复垦土壤施肥管理方式。 |
| 关键词: 氮肥形态；固氮菌；溶解性总氮；复垦土壤；土壤酶活性 |
| DOI：10.13522/j.cnki.ggps.2022153 |
| 分类号: |
| 基金项目: |
|
| Efficacy of Nitrogen-fixing Bacteria Combined with Different Nitrogen Fertilizers in Improving Enzymatic Activity and Nitrogen in Reclaimed Soil |
|
WANG Shuaibing, MENG Huisheng, WU Xin, ZHANG Jie, HAO Xianjun, HONG Jianping, JIAO Jincheng
|
|
College of Resources and Environment, Shanxi Agricultural University, Taigu 030801, China
|
| Abstract: |
| 【Objective】Reclaimed soils are usually poor in nutrients and the aim of this paper is to investigate the efficacy of nitrogen-fixing bacteria combined with different nitrogen fertilizations in improving fertility of reclaimed soils. 【Method】The experiments were conducted in pots filled with soil collected from a subsidized coal mining. The soil was incubated with nitrogen-fixing bacteria combined with different nitrogen fertilizers. In each treatment, we measured carbon and nitrogen in microbial biomass, enzymatic activity, total nitrogen, and ammonium and nitrate nitrogen in the soil.【Result】Combining nitrogen-fixing bacteria with ammonium or nitrate nitrogen fertilizer increases carbon and nitrogen in microbial biomass, total dissolved nitrogen, ammonium nitrogen in the soil. Compared with treatment with nitrate and ammonium nitrogen fertilization only, their combination with nitrogen-fixing bacteria increases the activity of catalase, sucrase, protease and urease by 4.96%, 17.85%, 12.53% and 6.12% respectively. Correlation analysis shows a close relationship between soil nutrients, enzymatic activity and carbon and nitrogen in microbial biomass; the activity of sucrase, protease and urease is positively correlated with total nitrogen, total dissolved nitrogen and ammonium nitrogen in the soil (P<0.01); the enzymatic activity is positively correlated with nitrate nitrogen (P<0.05); a positive correlation exists between carbon and nitrogen in microbial biomass and total dissolved nitrogen in the soil (P<0.05). Principal component analysis shows that ammonium nitrogen fertilization combined with nitrogen-fixing bacteria gives the best soil quality.【Conclusion】Combining nitrate and ammonium nitrogen fertilization with nitrogen-fixing bacteria can significantly increase soil microbial biomass and nitrogen content, and it can be used as an improved agricultural practice to improve quality of reclaimed soil from coal mining. |
| Key words: nitrogen fertilizer forms; nitrogen-fixing bacteria; dissolved total nitrogen; reclaimed soil; soil enzyme activity |
