| 引用本文: | 田德龙,侯晨丽,徐 冰,等.分枝期水盐胁迫对紫花苜蓿生长及生理特征的影响[J].灌溉排水学报,2023,42(4):8-14. |
| TIAN Delong,HOU Chenli,XU Bing,et al.分枝期水盐胁迫对紫花苜蓿生长及生理特征的影响[J].灌溉排水学报,2023,42(4):8-14. |
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| 摘要: |
| 【目的】探讨分枝期水盐胁迫对紫花苜蓿生长及生理特征的影响。【方法】以紫花苜蓿为研究对象，于2018—2019年在内蒙古磴口县圣牧高科经济园区内开展盆栽试验，设置3个水分胁迫处理：W1（55%θf ~70%θf）、W2（70%θf ~85%θf）、W3（85%θf ~100%θf），并设置4个盐分胁迫处理：S0（<1.0 g/kg）、S1（1.5 g/kg）、S2（3.0 g/kg）、S3（4.5 g/kg），在苜蓿初花期测量各处理的地上部分、根系生物量以及地上部分植株渗透调节物质和抗氧化酶活性，探究水盐胁迫对紫花苜蓿水分消耗、生长及生理特性的影响。【结果】与单一水分和盐分胁迫相比，水盐胁迫导致苜蓿耗水量和地上生物量进一步降低。在低水处理下，添加适宜的盐分（≤3 g/kg）可提高苜蓿自身的脯氨酸量、Na+量以及过氧化物酶（POD）活性，降低超氧化物歧化酶（SOD）活性，缓解干旱对苜蓿的氧化损害，促进苜蓿生长。其中，S2W1处理下的茎叶脯氨酸量、Na+量及POD活性相比S0W1处理提高74.28%、17.28%和34.60%，SOD活性减少9.87%，耗水量、地上部分生物量相比S0W1处理分别增加6.48%和2.17%。【结论】干旱和盐分复合胁迫条件下，紫花苜蓿表现出一定的交叉适应现象，盐分质量分数在2~3 g/kg、灌水量在田间持水率的70%~85%时，最适宜苜蓿生长。 |
| 关键词: 水盐胁迫；紫花苜蓿；生物量；渗透调节物质；抗氧化酶活性 |
| DOI：10.13522/j.cnki.ggps.2022403 |
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| Combined Effect of Water and Salt Stress on Growth and Physiological Traits of Alfalfa at Branching Stage |
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TIAN Delong, HOU Chenli, XU Bing, REN Jie, ZHANG Chen
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1. Institute of Water Resources for Pastoral Area, Ministry of water Resources, Huhhot 010020, China;
2. School of Energy and Environment, Inner Mongolia University of Science and Technology, Baotou 014010, China
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| Abstract: |
| 【Objective】Alfalfa is sensitive to water stress at branching stage, but how a simultaneous salinity stress affects this sensitivity is not well understood. The objective of this paper is to address this issue.【Method】Pot experiments were conducted in 2018—2019 at the Shengmu High-tech Economic Park, in Dengkou County, Inner Mongolia. It consisted of three water treatments with the soil water content kept at 55%~70% (W1), 75%~85% (W2), and 85%~100% (W3) of the field capacity, respectively. Each water treatment had four salinity stresses with the soil salt content set at 1.0 (S0), 1.5 (S1), 3.0 (S2) and 4.5(S3) g/kg, respectively. In each treatment, we measured osmotic substances, antioxidant enzymatic activities, above- and below-ground biomass at early flowering stage. 【Result】Water and salinity stresses reduced water consumption and aboveground biomass more considerably when they worked collectively than working separately. When the water stress was minor, however, imposing a slight salinity stress with soil salt content ≤3 g/kg can increase the contents of proline and Na+, boosted peroxidase (POD) activity, and reduced superoxide dismutase (SOD). This alleviated oxidative damage of the water stress, thereby promoting crop growth. Compared with S0+W1, S2+W1 increased the contents of proline and Na+, and boosted POD activity, by 74.28%, 17.28% and 34.60%, respectively, while reducing SOD activity by 9.87%. These led to an increase in water consumption and aboveground biomass by 6.48% and 2.17%, respectively.【Conclusion】When water stress is not severe, imposing a slight salinity stress can improve the tolerance of alfalfa to water and salinity stress at branching stage. For all treatments we studied, keeping soil salt content in the range of 2~3 g/kg and soil water content in the range of 70%~85% of the field water capacity was optimal to improve resistance of the crop to both salinity and water stresses. |
| Key words: water salt stress; alfalfa; biomass; osmotic regulating substance; antioxidant enzyme activity |
