| 引用本文: | 李晓婷,李立军,张永平,等.干旱胁迫下外源内生真菌对燕麦种子萌发及幼苗生长的影响[J].灌溉排水学报,2023,42(7):10-17. |
| LI Xiaoting,LI Lijun,ZHANG Yongping,et al.干旱胁迫下外源内生真菌对燕麦种子萌发及幼苗生长的影响[J].灌溉排水学报,2023,42(7):10-17. |
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| 摘要: |
| 【目的】探讨外源内生真菌对干旱胁迫下燕麦种子萌发和幼苗生长的影响。【方法】以燕麦（Avena sativa L.）为研究对象，设正常水分处理（WW）和12%PEG-6000模拟干旱胁迫（HD）2个水分处理，分别添加Czapek培养基（CK）、外源内生真菌O-2（Fusarium equiseti）和O-66（Alternaria pomicola），分析干旱胁迫下外源内生真菌对种子发芽、幼苗生长、根系结构和生理特性的影响。【结果】外源内生真菌O-2、O-66均能在燕麦根中检测到。干旱胁迫显著抑制了种子的萌发，内生真菌O-2和O-66均提高了种子发芽率和发芽势，其中添加O-66处理发芽率和发芽势较CK显著增加了44.68%和41.18%。内生真菌显著促进胚根和胚芽的伸长，分别是CK的1.15~1.36倍和1.18~1.42倍。水培试验中，干旱胁迫下内生真菌均提高了燕麦幼苗的株高、干物质积累量和根系指标，其中添加O-66处理的株高和冠层干质量增加了23.67%和39.58%，根干质量、根系总长度、根系投影面积、根系总表面积、根系总体积和根尖数分别增加了33.72%、20.40%、35.47%、31.84%、36.94%和59.24%。与CK相比，干旱胁迫下内生真菌处理的燕麦根系活力显著提高了15.34%~23.54%，SOD、POD和CAT分别提高了25.97%~30.48%、14.26%~20.47%和37.46%~45.72%，MDA量降低了9.27%~10.33%。【结论】外源内生真菌能显著缓解干旱胁迫对燕麦种子及幼苗的伤害，增强植株耐旱性，促进燕麦生长，其中内生真菌O-66的抗旱效果较为明显。 |
| 关键词: 干旱胁迫；内生真菌；种子萌发；幼苗；根系形态；生理特性 |
| DOI：10.13522/j.cnki.ggps.2022697 |
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| Using Exogenous Endophytic Fungi to Improve Seed Germination and Seedling Development of Oat under Water Stress |
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LI Xiaoting, LI Lijun, ZHANG Yongping, GUO Yunfei, HAN Xue
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College of Agriculture, Inner Mongolia Agricultural University, Hohhot 010019, China
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| Abstract: |
| 【Objective】Seed germination and its subsequent seedling development underpin the ultimate yield, but they are prone to water stress. This paper studies the feasibility and effectiveness of using exogenous endophytic fungi to boost germination of oat (Avena sativa L.) seed and its seedling development under drought conditions. 【Method】Oat seeds were treated with tap water (WW); water stress in the petri dishes was created by adding 12% of PEG-6000 to the water. The Czapek medium was used as the control (CK). The seeds were inoculated by endophytic fungi O-2 (Fusarium equiseti) and O-66 (Alternaria pomicola), respectively. In each treatment, we measured seed germination and the subsequent seedling growth, morphology, and physiological traits of the roots. 【Result】Successful colonization of the exogenous endophytic fungi O-2 and O-66 was identified in the oat roots. Drought stress significantly inhibited seed germination, but inoculation with endophytic fungi O-2 and O-66 increased the germination rate. Notably, inoculation with fungus O-66 significantly increased the germination rate and seedling development by 44.68% and 41.18%, respectively, compared to CK. The endophytic fungi also promoted radicle and germ elongation, which were 1.15 to 1.36 times and 1.18 to 1.42 times that of CK, respectively. In hydroponic culture, all endophytic fungi enhanced plant height, dry matter accumulation, and root index of the oat seedlings. O-66 inoculation resulted in a 23.67% increase in plant height and a 39.58% increase in canopy dry weight. Additionally, inoculation also increased root dry weight, total root length, projected root area, total root surface area, total root volume and root tips significantly by 33.72%, 20.40%, 35.47%, 31.84%, 36.94% and 59.24%, compared to CK. Inoculation with endophytic fungi increased root activity, and elevated SOD, POD, and CAT enzymes by 25.97% to 30.48%, 14.26% to 20.47% and 37.46% to 45.72%, respectively. Compared to CK, inoculation reduced the content of MDA (malondialdehyde), a marker of oxidative stress, by 9.27% to 10.33%. 【Conclusion】Inoculating the oat seeds by exogenous endophytic fungi can effectively alleviate drought stress to their germination and the subsequent seedling development, especially when inoculated with the fungus O-66. These results provide valuable insight into the use of endophytic fungi to mitigate drought stress to seed germination and improve crop growth. |
| Key words: drought stress; endophytic fungi; seed germination; seedlings; root morphology; physiological property |
