| 引用本文: | 邱虎森,刘杰云,张文正,等.生物质炭和石灰氮削弱温室土壤镰刀菌与真菌群落关联度[J].灌溉排水学报,2023,42(10):114-121. |
| QIU Husen,LIU Jieyun,ZHANG Wenzheng,et al.生物质炭和石灰氮削弱温室土壤镰刀菌与真菌群落关联度[J].灌溉排水学报,2023,42(10):114-121. |
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| 摘要: |
| 【目的】了解不同农艺措施对温室土壤真菌多样性及土壤真菌与镰刀菌菌群的相互关系的影响，探寻设施农业土壤质量提升的绿色改良措施。【方法】通过培养试验研究了单施或混合施入生物质炭和石灰氮对土壤理化、真菌群落结构和镰刀菌属丰度差异及关系。【结果】与对照相比，单施生物质炭显著提高了土壤pH值和速效钾（P<0.05）；单施石灰氮有利于土壤pH值、铵态氮和硝态氮的提高（P<0.05）；生物质炭和石灰氮混合施入显著提高土壤pH值、电导率、铵态氮、硝态氮量和速效钾量（P<0.05）。与对照相比，单施生物质炭或生物质炭与石灰氮的混合施入均显著降低土壤真菌群落香农多样性指数（P<0.05）。各处理真菌群落优势菌门相对丰度差异不显著。生物质炭与石灰氮混合施入后土壤镰刀菌属的相对丰度显著低于对照（P<0.05）。土壤镰刀菌属真菌多样性与土壤电导率、铵态氮量、真菌群落香农多样性指数均呈显著正相关（P<0.05）。基于网络分析结果表明，与对照相比，单施或混施生物质炭和石灰氮均能降低镰刀菌群与真菌菌群关系的复杂性。【结论】生物质炭与石灰氮的混合施入增强了镰刀菌菌群与真菌菌群的竞争关系，是一种有利于缓解温室土壤病害的较好措施。 |
| 关键词: 温室；生物质炭；石灰氮；镰刀菌属；真菌群落多样性 |
| DOI：10.13522/j.cnki.ggps.2023146 |
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| Amending Greenhouse Soil Using Biochar and Lime Nitrogen Reduces the Correlation between Fungal and Fusarium Communities |
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QIU Husen, LIU Jieyun, ZHANG Wenzheng, LYU Mouchao, WANG Yu
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1. Farmland Irrigation Research Institute, Chinese Academy of Agricultural Sciences, Xinxiang 453002, China;
2. School of Environment and Surveying Engineering, Suzhou University, Suzhou 234000, China
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| Abstract: |
| 【Objective】Biochar and lime nitrogen have been increasingly utilized as conditioners to improve soil quality, yet their effects on soil microbial communities remain relatively unexplored. This study aims to investigate the impact of soil amendment with biochar and lime nitrogen on fungal community diversity and the correlation between fungi and Fusarium. 【Method】The experiment was conducted in soil columns with biochar and lime nitrogen applied separately or in combination. Untreated soil served as the control. For each treatment, we measured physicochemical properties, fungal community structure, Fusarium abundance in the soil, as well as their interactions. 【Result】In comparison to the control, application of biochar significantly increased soil pH and available potassium (P<0.05). Application of lime nitrogen alone increased pH, ammonium nitrogen, and nitrate nitrogen in the soil (P<0.05). Combined application of biochar and lime nitrogen significantly increased pH, electrical conductivity, ammonium nitrogen, nitrate nitrogen, and available potassium of the soil (P<0.05). It was found that applying biochar alone or in combination with lime nitrogen significantly reduced the fungal Shannon diversity index (P<0.05), although there were no significant differences in the relative abundance of the dominant fungal phylum between the treatments. Additionally, combined application of biochar and lime nitrogen led to a significant reduction in the relative abundance of Fusarium (P<0.05). The Mantel-Haenzel test revealed significant positive correlations between the Fusarium community and soil conductivity, ammonium nitrogen, and aroma diversity of the fungal community (P<0.05). Network analysis showed that separate or combined application of biochar and lime nitrogen reduced the number of both positive and negative connections and nodes between the Fusarium community and fungal community. 【Conclusion】The combined application of biochar and lime nitrogen enhanced the competition between the Fusarium community and the fungal community, which is beneficial for mitigating soil pathogens. |
| Key words: greenhouse; biochar, lime nitrogen; Fusarium; fungal community diversity |
