冰封期富营养化湖泊细菌菌群结构特征分析

杨文焕; 毋凡琛; 李卫平; 张 生

引用本文:	杨文焕,毋凡琛,李卫平,等.冰封期富营养化湖泊细菌菌群结构特征分析[J].灌溉排水学报,0,():-.
	YANG Wen-huan,WU Fan-chen,LI Wei-ping,et al.冰封期富营养化湖泊细菌菌群结构特征分析[J].灌溉排水学报,0,():-.

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冰封期富营养化湖泊细菌菌群结构特征分析
杨文焕,毋凡琛,李卫平,等
1.内蒙古农业大学水利与土木建筑工程学院;2.内蒙古科技大学能源与环境学院

摘要:

【目的】为探究寒旱区湖泊冰封期细菌菌群结构特征及影响因素，【方法】采用高通量测序技术对南海湖不同湖区12个采样点水体及沉积物细菌进行测定，分析多样性指数、细菌群落组成后进行相关性热图分析。【结果】不同区域物种多样性有一定差异。从门水平来看，细菌主要为变形菌门（Proteobacteria）、绿弯菌门（Chloroflexi）、蓝藻门（Cyanobacteria）、拟杆菌门（Bacteroidetes）和放线菌门（Actinobacteria）。沉积物中以变形菌门（40%）和绿弯菌门（24%）居多，水体中以蓝藻门（29%）和放线菌门（33%）居多，菌群丰度在旅游开发区较大。从属水平分析，优势类群集中在硫杆菌属（thiobacillusThiobacillus）、未分类蓝细菌科（unclassifiednorank_-c_-Cyanobacteria）、未分类绿弯菌科（unclassified_-p_-Chloroflexi）。沉积物中以硫杆菌属居多，约占21%，水体中以未分类蓝细菌科居多，约占24%，其在4个区域均有大量分布，旅游开发区最多。TN、DO、T对水体细菌群落结构影响较大，TN、NH4+-N、C/N对沉积物细菌群落结构影响较大，控制其量是控制南海湖水华爆发的重要条件。【结论】南海湖在冰封期富营养化加剧，在各区域沉积物中均有Thiobacillus，其在南海湖冰封期的生物地球化学循环中扮演着重要的角色，对预防黑臭水体发生具有重要作用。

关键词: 细菌;群落结构;高通量测序;南海湖;冰封期

DOI：

分类号:X172;X524

基金项目:内蒙古自然科学基金(2018LH04002)；国家重点研发计划（2019YFC0609204）

Analysis on the Structural Characteristics of Bacteria in Eutrophication Lake during the Ice Period

YANG Wen-huan¹, WU Fan-chen², LI Wei-ping², ZHANG Sheng^1,3,4

1.Institute of Water Conservancy and Civil Engineering,Inner Mongolia Agricultural University;2.The Colloge of Environment Energy Resources,Inner Mongola University of Science Technology,Baotou;3.The Colloge of Environment Energy Resources,Inner Mongola University of Science Technology,Baotou,;4.China

Abstract:

【Objective】To explore the diversity of bacterial communities in Baotou Nanhai Lake of cold-arid area in ice period,【Method】the surficial sedimentary bacterial of 12 sampling sites in different areas was sequenced using high-throughput sequencing approach and then correlation thermogram analysis was performed.【Result】The result showed that the diversity of species in different areas was quite different. At the phyla level, Proteobacteria, Chloroflexi, Cyanobacteria, Bacteroidetes and Actinobacteria were dominant. The number of Proteobacteria and Chloroflexi were more than others in the sediment, Cyanobacteria (29%) and Actinobacteria (33%) were dominant in the water. The abundance of bacteria was higher in the tourism development zone. Thiobacillus, norank-f- Cyanobacteriaanaerolineaceae, unclassified-p-Chloroflexi were dominant bacteria at the genus level. Thiobacillus (21%) was higher in the sediment and norank-f- Cyanobacteriaanaerolineaceae (24%)was higher in the water, which were widely distributed in the four regions. The redundancy analysis for the relationship between bacterial communities and environmental factors revealed that the TN、DO and T had great influence on the structure of bacterial community in the water, TN、NH4+-N and COD were the same influence in the sediment, Controlling the content of these elements is an important condition for controlling the outbreak of bloom in Nanhai Lake.【Conclusion】The eutrophication of Nanhai Lake increased during the ice period, and Thiobacillus that was found in the sediments of all regions played an important role in biogeochemical cycles of sediments in Nanhai Lake. It is of great importance for preventing the occurrence of black and odorous water.

Key words: bacteria; community structure; high-throughput sequencing; Nanhai Lake; ice period