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引用本文:陈碧华,郭卫丽,孟凡茹,等.镉胁迫对南瓜植株镉吸收积累及光合特性影响[J].灌溉排水学报,2020,(12):-.
陈碧华,Guo weili,Meng fanru,et al.镉胁迫对南瓜植株镉吸收积累及光合特性影响[J].灌溉排水学报,2020,(12):-.
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镉胁迫对南瓜植株镉吸收积累及光合特性影响
陈碧华, 郭卫丽, 孟凡茹, 周俊国, 李新峥
河南科技学院园艺园林学院
摘要:
【目的】为了研究镉在南瓜植株中的吸收积累特性及镉胁迫对南瓜叶片光合作用的影响。【方法】采用微波消解-ICP-AES技术,以中国南瓜“盐砧一号”为试验材料,设置0、2、4、6 mg.L-1四个镉胁迫浓度的基质栽培试验,测定Cd在南瓜不同器官的分布、Cd的亚细胞分布以及镉胁迫下南瓜叶片的光合作用参数。【结果】Cd在南瓜植株根中含量远大于茎和叶,随着镉胁迫浓度增大,根中富集系数BCF 值呈上升趋势,转运系数 TF值逐渐降低。Cd在不同器官亚细胞组分中呈不均匀分布,其中根系中以细胞壁的分配率最高达到47.63%~57.69%;其次为细胞质;细胞器中Cd含量仅占1.93%~4.59%。镉胁迫没有对南瓜植株净光合速率(Pn)造成显著抑制作用,镉胁迫显著抑制了气孔导度(Gs) 和蒸腾速率(Tr) ,但镉胁迫对胞间CO2浓度(Ci)没有显著差异影响。【结论】中国南瓜“盐砧一号”在镉胁迫下能将Cd富集在根系的细胞壁中,从根部转移到茎和叶的能力减弱,对光合作用没有造成显著抑制作用。
关键词:  镉胁迫;南瓜;亚细胞分布;光合特性;富集系数;转移系数
DOI:
分类号:S642
基金项目:
Effects of Cadmium Stress on Cadmium Uptake Accumulation and Photosynthetic Characteristics of Pumpkin Plants
陈碧华, Guo weili, Meng fanru, Zhou junguo, Li xinzheng
Henan Institute of Science and Technology
Abstract:
【Objective】 In order to study the absorption and accumulation characteristics of cadmium in pumpkin plants and the effect of cadmium stress on photosynthesis of pumpkin leaves. 【Methods】Using microwave digestion-ICP-AES technology and the Chinese pumpkin "Yanzhen 1" as experimental material. Substrate cultivation experiment with four cadmium stress concentrations of 0, 2, 4, 6 mg.L-1. The photosynthetic parameters of leaves, the sub-cellular distribution of Cd and the distribution of organs (roots, stems and leaves) were determined during growth.【Results】The results showed that the content of Cd in pumpkin root increased much more than that of stem and leaf. With the increase of Cd concentration, the BCF value of root enrichment factor increased and the TF value of transport coefficient decreased gradually. Cd was unevenly distributed among the subcellular components of different organs. The distribution rate of cell wall in roots was up to 47.63%~57.69%, followed by cytoplasm; Cd content in organelles only accounted for 1.93%~4.59%. Cd treatment did not significantly inhibit the net photosynthetic rate (Pn) of pumpkin, while Cd treatment significantly inhibited stomatal conductance (Gs) and transpiration rate (Tr), but there was no significant difference in intercellular CO2 concentration (Ci) between Cd treatment. 【Conclusion】Under the cadmium stress, the Chinese pumpkin “Yanzhen 1” could enrich Cd in the cell wall of the root system, and its ability to transfer from the root to the stem and leaves is weakened, which does not cause significant inhibition on photosynthesis.
Key words:  Cadmium Stress; Pumpkin;subcellular disposition;photosynthetic;concentration factor; Transfer Factor