盐胁迫对棉花幼苗叶片叶绿素荧光参数的影响

孙文君; 江晓慧; 付媛媛; 申孝军; 高阳; 王兴鹏

引用本文:	孙文君,江晓慧,付媛媛,等.盐胁迫对棉花幼苗叶片叶绿素荧光参数的影响[J].灌溉排水学报,0,():-.
	SUN Wenjun,JIANG Xiaohui,FU Yuanyuan,et al.盐胁迫对棉花幼苗叶片叶绿素荧光参数的影响[J].灌溉排水学报,0,():-.

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盐胁迫对棉花幼苗叶片叶绿素荧光参数的影响
孙文君¹, 江晓慧², 付媛媛^1,3, 申孝军³, 高阳³, 王兴鹏¹
1.塔里木大学水利与建筑工程学院;2.山东农业大学水利土木工程学院;3.农业农村部作物需水与调控重点实验室/中国农业科学院农田灌溉研究所

摘要:

【目的】研究盐分胁迫对棉花幼苗叶片叶绿素荧光特性的影响，为了解盐分胁迫抑制棉花幼苗生理过程提供参考。【方法】在人工气候室内以“新陆中37”为供试材料，分析了50、100、150和200 mmol·L-1 4 个盐分浓度下棉花叶片叶绿素荧光参数的变化特征。【结果】结果表明，50 mmol·L-1盐分浓度处理的棉花叶片叶绿素相对含量（SPAD）、最大荧光（Fm）、最小荧光（Fo）、最大量子效率（Fv/Fm）、实际量子效率（Y(II)）和光化学淬灭（qP））等参数值与对照接近或略低于对照，但100~200 mmol·L-1盐分浓度处理的SPAD、Fv/Fm、Y(II)等参数则显著降低；非光化学淬灭参数qN在各盐分处理间的差异不显著，但高盐分处理的过剩光能（(1-qP)/NPQ）和非光化学淬灭参数NPQ则显著高于对照处理，但低盐分浓度处理与对照间的差异不显著。【结论】棉花在低浓度50~100 mmol·L-1盐分处理浓度范围里通过自身的调节机制能够避免光反应系统受到损伤，而当盐分浓度达到150 mmol·L-1时，引发光合电子传递受阻，棉花叶片受到光抑制，导致叶片的光能利用效率显著降低，最终导致植物生长受到抑制。

关键词: 棉花幼苗;盐胁迫;荧光参数，叶绿素相对含量

DOI：

分类号:S562;Q945.78

基金项目:国家自然科学基金重大项目课题：棉花水盐响应与根区动态阈值(51790534); 国家重点研发计划课题：棉花非充分灌溉制度及节水控盐高效灌溉模式(2016YFC0400208)；

Effects of salt stress on chlorophyll fluorescence parameters of cotton seedling leaves

SUN Wenjun¹, JIANG Xiaohui², FU Yuanyuan^1,3, SHEN Xiaojun³, GAO Yang³, WANG Xingpeng¹

1.Tarim University;2.School of Hydraulic and Civil Engineering, Shandong Agricultural University;3.Key laboratory of crop water demand and regulation, ministry of agriculture and rural affairs/institute of farmland irrigation

Abstract:

【Objective】To study the effects of salt stress on chlorophyll fluorescence characteristics of cotton seedling leaves, and provide a reference for understanding the physiological process of salt stress inhibiting cotton seedling. 【Method】The chlorophyll fluorescence parameters of cotton leaves were analyzed under four salt concentrations of 50, 100, 150 and 200 mmol . L^-1 with "XinluZhong-37" as the test material. 【Result】Results showed that the relative chlorophyll content (SPAD), maximum fluorescence (Fm), minimum fluorescence (Fo), maximum quantum efficiency (Fv / Fm), actual quantum efficiency (Y (II)) and photochemical quenching (QP)) of cotton leaves in the treatment of 50 m mol L^-1 were close to or lower than those values in CK, while the corresponding parameters in 100-200 m mol L^-1 were significantly lower than CK. There was no significant difference in the non-photochemical quenching parameter of qN among different treatments. However, the excess light energy ((1-qp) / NPQ) and non-photochemical quenching parameter of NPQ in the treatments with high salt concentration were markedly greater than the values in CK, the difference in (1-qp) / NPQ and NPQ between the low salt concentration treatment and CK was insignificant. 【Conclusion】Cotton can avoid the damage of light reaction system through its own regulation mechanism in the low salt concentration of 50 ~ 100 mmol . L^-1. When the salt concentration reaches 150 mmol . L^-1, the light emission and electron transfer of cotton leaves are blocked, resulting in the significant decrease of light energy utilization efficiency of cotton leaves, and finally limiting the plant growth.

Key words: Cotton seedling; Salt stress; Fluorescence parameters; Relative chlorophyll content