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引用本文:李焕弟,缴锡云,李江.基于MICP及EICP技术的土壤固化试验研究[J].灌溉排水学报,0,():-.
LIHUANDI,JIAOXIYUN,LIJIANG.基于MICP及EICP技术的土壤固化试验研究[J].灌溉排水学报,0,():-.
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基于MICP及EICP技术的土壤固化试验研究
李焕弟, 缴锡云, 李江
河海大学农业科学与工程学院
摘要:
【目的】针对传统土质边坡固化方法操作复杂、维修维护困难问题,开展基于微生物诱导碳酸钙沉积(MICP)及脲酶诱导碳酸钙沉积(EICP)技术的土壤固化研究,为农田土质排水沟边坡生态防护提供一定的理论指导。【方法】基于MICP及EICP技术的土壤固化研究,使用巴氏芽孢杆菌和脲酶进行土壤固化试验,设置巴氏芽孢杆菌(菌组)、脲酶(脲酶组)处理,通过溶液试验,对比研究巴氏芽孢杆菌菌液、脲酶溶液分别与固结液(尿素/氯化钙混合液)以不同配比(1:3、2:3、1:1、4:3、5:3)混合下的碳酸钙生成量,并进行土壤多轮次喷洒试验,分析不同固化轮次下土壤表面形态、碳酸钙量和微观结构特征,探讨巴氏芽孢杆菌、脲酶对土壤的固化效果。【结果】巴氏芽孢杆菌、脲酶作为MICP和EICP过程的催化剂水解尿素,然后与氯化钙生成碳酸钙晶体,不同催化剂与固结液的最佳配比为菌组2:3、脲酶组5:3,其碳酸钙产率分别为86.7%、84.1%;两处理以2:3配比对土壤进行多轮次喷洒,生成的碳酸钙可有效修复土壤表层裂缝,菌组碳酸钙量比脲酶组高,但其差距在后期逐渐减小;巴氏芽孢杆菌和脲酶诱导产生的碳酸钙晶体形态不同。【结论】巴氏芽孢杆菌、脲酶诱导沉积的碳酸钙晶体填充在土颗粒之间,可达到良好的土壤固化效果,本研究条件下,巴氏芽孢杆菌处理效果比脲酶处理好。。
关键词:  巴氏芽孢杆菌;脲酶;土壤固化;碳酸钙量;微观结构
DOI:
分类号:S276.3
基金项目:宁夏回族自治区重点研发计划(2018BBF02022);中央高校基本科研业务费专项资金资助(B210202120);清华大学水沙科学水利水电工程国家重点实验室及宁夏银川水联网数字治水联合研究院联合开放研究基金(sklhse-2020-Iow02)
Experimental Study on Soil Cementation Based on MICP and EICP Technology
LIHUANDI, JIAOXIYUN, LIJIANG
College of Agricultural Science and Engineering, Hohai University
Abstract:
【Background】Farmland drainage ditch is an important guarantee of agricultural safety in China. For the protection of farmland drainage ditch, soil hardening treatment, material improvement measures and other methods are not only high cost, but also difficult to maintain later, which may have a certain adverse impact on the environment. And a new soil cementation technology is urgently needed. In terms of farmland drainage ditch cementation, the technique of calcium carbonate precipitation induced by microorganism and urease is easy to operate, and the cementation effect can be controlled, and there is no negative impact on the environment. The use of the technique is an effective method to improve drainage ditch in constructing sustainable agriculture.【Objective】The ecological construction of farmland drainage ditch is conducive to the development of agriculture. The traditional soil cementation technology is complex in operation and difficult to maintain. The technology of Sporosarcina pasteurii and urease induced calcium carbonate precipitation can not only meet the requirements of soil cementation but also meet the ecological requirements. This technology can cement soil particles into a whole and effectively improve the stability of farmland drainage ditches. The purpose of this paper is to study the soil cementation effect based on MICP and EICP, Sporosarcina pasteurii and urease were used to catalyze urea hydrolysis to induce calcium carbonate deposition, so as to apply it to farmland drainage ditch, improve the stability of drainage ditch slope and its environmental friendliness.【Method】Sporosarcina pasteurii group and urease group were set up in this study. Sporosarcina pasteurii and urease were mixed with the cementation solution (urea-calcium chloride solution) in different proportions to explore the difference of calcite content between them. The ratio of Sporosarcina pasteurii solution and cementation solution was 1:3, 2:3, 1:1, 4:3 and 5:3. The ratio of urease solution was the same as that of Sporosarcina pasteurii solution and cementation solution. Furthermore, experiments were conducted for cementing soil by spraying microbial solution/urease solution and the cementation solution to the surface of soil. The soil surface morphology, content of calcite and soil microstructure under different spray round times were performed to compare the difference of Sporosarcina pasteurii and urease.【Result】Sporosarcina pasteurii and urease can be used as catalysts for MICP and EICP technology to hydrolyze urea and form calcium carbonate crystals with calcium chloride solution. When the ratio of microbial solution and cementation solution is 2:3, the effect is better. However, when the ratio of urease solution and cementation solution is 5:3, the better effect can be obtained.The highest calcium yield of Sporosarcina pasteurii group and urease group was 86.7%, 84.1%, respectively. After spraying the soil with the ratio of 2:3 for several times, surface cracks in the soil are repaired and the calcite produced by the microbial treatment is higher than that of the urease treatment, the difference between the two treatments decreased gradually in the later stage. Calcium carbonate crystals formed by Sporosarcina pasteurii and urease are different.【Conclusion】Sporosarcina pasteurii and urease catalyze the hydrolysis of urea to form calcite, which can be filled between soil particles to achieve good soil cementation effect. Under the condition of this study, the cementation effect of Sporosarcina pasteurii treatment slightly better than that of urease treatment.
Key words:  Sporosarcina pasteurii; urease; soil cementation; calcite content; microstructure