| 引用本文: | 王帅兵,孟会生,武欣,等.不同形态氮肥配施固氮菌对复垦土壤酶活性及氮素形态的影响[J].灌溉排水学报,2022,():-. |
| wangshuaibing,menghuisheng,wuxin,et al.不同形态氮肥配施固氮菌对复垦土壤酶活性及氮素形态的影响[J].灌溉排水学报,2022,():-. |
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| 摘要: |
| 【目的】为探究固氮菌与不同形态氮肥配施能否发挥更大效能以及寻找矿区土地复垦的最佳施肥方式，【方法】选取采煤塌陷区复垦1a的土壤为研究对象，通过盆栽试验分析各形态氮肥与固氮菌配施对土壤微生物量碳氮、酶活性、全氮、溶解性总氮、铵态氮、硝态氮含量的影响。【结果】硝铵态氮肥配施固氮菌可有效提高复垦土壤微生物量碳氮、溶解性总氮、铵态氮含量，与硝铵态氮肥处理相比土壤过氧化氢酶、蔗糖酶、蛋白酶、脲酶活性分别显著提高4.96%、17.85%、12.53%、6.12%。相关分析表明土壤养分、酶活性和微生物量碳氮之间关系密切，蔗糖酶、蛋白酶、脲酶与土壤全氮、溶解性总氮、铵态氮均呈极显著正相关(P< 0.01)，与硝态氮呈显著正相关(P< 0.05)，土壤微生物量碳、氮均与溶解性总氮呈显著正相关(P< 0.05)，可用来评价复垦土壤肥力状况。主成分分析进一步表明，不同施肥处理复垦土壤质量以硝铵态氮肥+固氮菌处理最高。【结论】硝铵态氮肥配施固氮菌更有利于提高土壤微生物活性及氮素含量，作为一种合理的施肥管理方式改善复垦土壤生态环境。 |
| 关键词: 氮肥形态;固氮菌;溶解性总氮;复垦土壤;土壤酶活性 |
| DOI： |
| 分类号:S154.2 S153.6 |
| 基金项目:(2017ZZ08)资助Supported by Project supported by the State Key Program of National Natural Science of China (U1710255、41907215) and Technology Innovation Fund of Shanxi Agricultural University (2017ZZ08) |
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| Effects of different forms of nitrogen fertilizer combined with nitrogen-fixing bacteria on soil enzyme activity and nitrogen forms in reclaimed soil |
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wangshuaibing, menghuisheng, wuxin, zhangjie, haoxianjun, hongjianping, jiaojincheng
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Shanxi Agricultural University
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| Abstract: |
| 【Objective】 In order to explore whether nitrogen-fixing bacteria combined application with different forms of nitrogen fertilizer can play a greater efficiency in reclaimed soil and the optimum fertilization method for land reclamation in mining area,【Method】 selected reclaimed 1a soil from coal mining subsidence area as the research object, a pot experiment was conducted to analyze the effects of different forms of nitrogen fertilizer combined with nitrogen-fixing bacteria on soil microbial biomass carbon and nitrogen, enzyme activity, total nitrogen, total dissolved nitrogen, ammonium nitrogen and nitrate nitrogen.【Result】 It showed that ammonium and nitrate nitrogen fertilizer combined with nitrogen-fixing bacteria could increase the contents of soil microbial biomass carbon and nitrogen, total dissolved nitrogen and ammonium nitrogen in reclaimed soil. Compared with the treatment of nitrate and ammonium nitrogen fertilizer, the activities of catalase, sucrase, protease and urease notably increased by 4.96%, 17.85%, 12.53% and 6.12% respectively. Correlation analysis indicated that there was a close relationship between soil nutrients, enzyme activity and soil microbial biomass carbon and nitrogen, which can be used to evaluate the fertility of reclaimed soil. The activity of sucrase, protease and urease were extremely significantly positive correlated with soil total nitrogen, total dissolved nitrogen and ammonium nitrogen (P < 0.01). The soil enzyme activity above was significantly positive correlated with nitrate nitrogen (P < 0.05). And there was also a significantly positive correlation between soil microbial biomass carbon and nitrogen and total dissolved nitrogen (P < 0.05). Principal component analysis further showed that the quality of reclaimed soil of different fertilized treatments was highest in the treatment of ammonium nitrogen fertilizer combined with nitrogen-fixing bacteria.【Conclusion】 The application of nitrate and ammonium nitrogen fertilizer combined with nitrogen-fixing bacteria was more beneficial to increase soil microbial characteristics and nitrogen content, as a reasonable fertilizer management way to improve the ecological environment of reclaimed soil. |
| Key words: nitrogen fertilizer forms; nitrogen-fixing bacteria; dissolved total nitrogen; reclaimed soil; soil enzyme activity |
