| Cite this article: | 邱虎森,刘杰云,甄博,等.涝渍胁迫对土壤无机氮及氨氧化微生物的短期效应[J].灌溉排水学报,0,():-. |
| Qiu Hu-Sen,Liu Jie-Yun,Zhen Bo,et al.涝渍胁迫对土壤无机氮及氨氧化微生物的短期效应[J].灌溉排水学报,0,():-. |
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| DOI： |
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| Short-term effect of waterlogging stress on soil inorganic nitrogen and ammonia oxidizing microorganisms |
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Qiu Hu-Sen1, Liu Jie-Yun1, Zhen Bo2, Niu Qing-Lin2, Li Hui-Zhen2, Zhou Xin-Guo2
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1.Suzhou University;2.Farmland Irrigation Research Institute, Chinese Academy of Agricultural Sciences
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| Abstract: |
| 【Objective】Understanding the effects of waterlogging stress on the distribution of inorganic nitrogen and ammonia oxidizing microorganisms in rhizosphere and non-rhizosphere soil of wheat is helpful to provide reference for exploring the method, such as waterlogging control, remedial measure of agricultural management, to reduce the loss of nitrogen . 【Method】In this study, different waterlogging times (0d, 3d and 5d) were set in anthesis of wheat. The differences of soil inorganic nitrogen and copy numbers of ammonia oxidizing microorganisms at the end of surface waterlogging, subsurface waterlogging and 2 days after drainage were compared. 【Result】Compared to control, extension of surface waterlogging time promoted the accumulation of ammonium nitrogen and significantly decreased the content of nitrate nitrogen in wheat rhizosphere and non-rhizosphere soil (P<0.05). Treatment of surface waterlogging stress for 5 days significantly decreased the copy number of ammonia-oxidizing archaea (AOA-amoA) gene in rhizosphere soil in comparison with control during the whole processes. Compared to control, surface waterlogging stress increased the copy number of ammonia-oxidizing bacteria (AOB-amoA) gene, but decreased ammonia-oxidizing bacteria biomass after surface waterlogging stress.. Redundancy analysis showed that ammonium nitrogen and pH significantly affected the structure of ammonia oxidizing microorganisms in non-rhizosphere soil. Stepwise regression analysis showed that the contents of ammonium nitrogen and nitrate nitrogen were regulated by ammonia-oxidizing archaea during the waterlogging stress and recovery process in non-rhizosphere soil. While, in rhizosphere soil, decrease of soil water content was unfavorable for the accumulation of ammonium nitrogen, but increased the content of nitrate nitrogen. The increase of available phosphorous may accelerate the accumulation of ammonium nitrogen.【Conclusion】After short-term waterlogging stress, the contents of soil inorganic nitrogen will be recovery quickly, application of phosphate fertilizer may improve soil inorganic nitrogen in rhizosphere soil. |
| Key words: waterlogging stress; ammonium nitrogen; nitrate nitrogen; ammonium oxidation |
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