| Cite this article: | 邱虎森,甄博,周新国.高温对水稻根际细菌群落及功能代谢多样性的影响[J].灌溉排水学报,0,():-. |
| Qiu Hu-Sen,Zhen Bo,Zhou Xin-Guo.高温对水稻根际细菌群落及功能代谢多样性的影响[J].灌溉排水学报,0,():-. |
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| Effect of heat stress on rhizobacteria diversity and functional metabolism diversity of rice |
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Qiu Hu-Sen, Zhen Bo, Zhou Xin-Guo
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Farmland Irrigation Research Institute, Chinese Academy of Agricultural Sciences
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| Abstract: |
| In general, rice crop can resist heat stress by regulating physiological and biochemical processes. The metabolism processes affect soil microenvironment and the structure and function of microbial community especially in rizhosphere via changing root sediment morphology and content. Meanwhile, rhizospheric bacteria can change soil nutrient form by adjusting their functional metabolism process, and then affect rice root absorption of soil nutrient and crop defense ability to external environment stress. To make clear the response of rhizobacteria functional metabolism to environmental stress is important for better understanding of soil ecosystem service function and biogeochemistry cycle. 【Objective】 Response of gene expression profiles that define the physiological metabolic processes of rhizobacteria to heat stress were revealed by using high-throughput technique and functional prediction. 【Method】 Based on pot experiments, heat stress was conducted for 7 days in rice jointing stage. The bacterial flora analysis, gene annotation and function classification are performed in conjunction with the PICRUSt (Phylogenetic Information of Common by Construction of States) and high-throughput sequencing. 【Result】 Compared with the control, heat stress promotes the enrichment of relatively low abundance bacterial classes (such as Gemmatimonadetes, Planctomycetes, Latescibacteria, Spirochaetae, Microgenomates, and Candidatus_Berkelbacteria), and the bacterial diversity. Also, heat stress promotes the increases of the number and percentage of positive links (bacteria-bacteria) between taxa. Most of the transcripts of bacteria were derived from Proteobacteria, and followed by Acidobacteria, Actinobacteria, and chlorobi. Based on the COG functional classification database, the main active genes were those correlated with metabolism and cellular processes and signaling in both control and heat stress treatments. However, heat stress increased the relative abundance of genes that participated in cell motility, signal transduction mechanisms, intracellular trafficking, secretion, and vesicular transport, and cell wall/membrane/envelope biogenesis. While, heat stress decreased the relative abundance of genes that participated in amino acid transport and metabolism, lipid transport and metabolism, secondary metabolites biosynthesis, transport and catabolism, and transcription. 【Conclusion】 In conclusion, heat stress had no effect on the rhizobacteria flora, but increased bacterial Shannon diversity, strengthens the symbiotic relationship of bacterial flora, and significantly affected the gene expression involved in bacterial growth and death, metabolism, and protein synthesis. |
| Key words: heat stress; rice; rhizobacteria, physiological metabolism; functional genes. |
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