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Cite this article:陈郁,张清涛,袁利兵,等.不同灌溉方式对旱区葡萄脯氨酸及渗透胁迫相关基因的影响[J].灌溉排水学报,0,():-.
Chenyu,Zhang Qingtao,Yuan Libing,et al.不同灌溉方式对旱区葡萄脯氨酸及渗透胁迫相关基因的影响[J].灌溉排水学报,0,():-.
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DOI:
Effects of Different Irrigation Methods on Proline and Osmotic Stress Related Genes of Vitis vinifera L.cv. Moldova in Arid Region
Chenyu, Zhang Qingtao, Yuan Libing, Chen Tuo, Chen Xiaohong
SUN YAN-SEN University
Abstract:
【Objective】Drought has a significant impact on global ecosystems in the context of global climate change. While, the agricultural water use efficiency of China is about 43%. Martina proposed that if the irrigation water efficiency is moderately increased by 10%, that is, by 0.3% per year within 50 years, the urban surface water deficit can be reduced by about 2.618 billion m3, which can help 78% water-vulnerable cities and 236 million residents in the world. Therefore, it is important to improve irrigation efficiency.This study is aimed to evaluate the applicability of different irrigation methods by analysis and comparison the effects of "microfiber capillary core infiltration irrigation" (microfiber infiltration irrigation) and other regular irrigation methods on soil water content, leaf water potential, proline, osmotic stress-related gene expression and water use efficiency of Moldovan grapes under different irrigation methods and water gradient. 【Methods】As a new water-saving irrigation technology, ‘microfiber capillary infiltration irrigation’ (microfiber infiltration irrigation, MII), based on the principle of capillarity, supplies water to the root zone of plants through the infiltration irrigation device made of microfiber materials, so as to achieve the purpose of water-saving. ‘Microfiber’ material has excellent water absorption performance, so ‘microfiber infiltration irrigation’ would effectively improve water use efficiency and productivity. However, the effect of "microfiber infiltration irrigation" on proline and related genes in Moldovan grape (Vitis vinifera L. cv. Moldova) remains unknown. The experiment was conducted during the grape growing season from May to September in 2019, in Yuquanying Farm at the eastern foot of Helan Mountain in Ningxia province, the national wine origin protection area. The study set up six different treatments, that is, different irrigation methods (using the same amount of water), including conventional ditch irrigation (CK1), drip irrigation (D), 10 cm depth in soil using capillary MII (C); different water volume gradients for surface MII (microfiber placed on the soil surface), that is, microfiber surface infiltration irrigation (ordinary irrigation volume, CK2), low irrigation volume (1/3 of ordinary irrigation volume, W1) and medium irrigation volume (2/3 of ordinary irrigation volume, W2). By measuring soil water content, leaf water potential, fruit proline content and relative expression of P5CS genes, we assessed the condition of drought in 6 treated Moldovan grapevines.【Results】From May 18 to September 30, 2019, a total of 136 days of the trial, all treatments presented varying degrees of drought conditions. Under different irrigation methods, the days of drought in CK1 were the most, about 73% of the total number of growth days; C and D were 67% and 40%, respectively, and the yield, water use efficiency and irrigation water use efficiency were C>D>CK1. Moreover, in the middle and later stages of the experiment, the soil water content of C and D was always higher than that of CK1. Under different water gradients, there was little difference on the condition of grape drought. In terms of leaf water potential and proline, under different irrigation methods, the water potential of CK1 was less than D, about 11.8%, 18.1%, 25.5%, 71.2%, 18.9% and 2.6% less than D in all the six measurements, which means that CK1 was under more severe water stress than D; as a result, the content of proline in CK1 was greater than that in D in all the measurements. In four of the six samples, the leaf water potential of CK1 was the smallest, C was the second, and D was the largest. Under different water gradients, the leaf water potential was W2W2>CK2As an important factor affecting proline synthesis, the content of P5CS gene reflects the drought conditions of plants under different irrigation methods. The relative expression of P5CS gene in CK1 was significantly higher than those of D and C on August 4, 25 and 28, but there was no significant difference between CK1, D and C on July 25 and September 4.【Conclusions】 Under different irrigation methods, D has the best ability to maintain soil water content, and C has the most days with moderate soil relative water content, and the water use efficiency of D and C was higher than that of CK1, indicating that both microfiber infiltration irrigation and drip irrigation are better water-saving irrigation methods. Under different water gradients, the relative expression level of P5CS gene in W1 was the highest among the six treatments at some stages, indicating that grapes under this treatment were subjected to more serious water stress, but its yield and water use efficiency were higher. Therefore, "superfine fiber" infiltration irrigation is an optimal irrigation method, which shows advantages and application value in maintaining soil effective water content.
Key words:  Microfiber irrigation; water stress; Vitis vinifera L. cv. Moldova; proline; P5CS;water use efficiency