| Cite this article: | 王贵彦.小麦根际微生物群落结构和多样性对水分胁迫的响应[J].灌溉排水学报,2022,(10):-. |
| Wang Guiyan.小麦根际微生物群落结构和多样性对水分胁迫的响应[J].灌溉排水学报,2022,(10):-. |
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| Response of rhizosphere microbial community structure and diversity to water stress of winter wheat |
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Wang Guiyan
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Hebei Agricultural University
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| Abstract: |
| Abstract: :?"Jimai 22" was used to study the effects of water stress on rhizosphere soil microbial community structure and diversity and enzymatic activity at flowering stage of winter wheat. Four levels of water stress treatments (CK, 75%-80% field water capacity; L, 60%-65% field water capacity; M, 40%-45% field water capacity; S, 30%-35% field water capacity) were designed. Illumina high-throughput sequencing technology was used to analyze the composition and diversity of soil microbial community. The results showed that compared with CK, L treatment significantly increased soil sucrase activity and had the highest sucrase and urease activities, which were 51.62 mg?g-1? d-1 and 0.85 mg?g-1?d-1, respectively, which were significantly higher than that of M and S treatments.?The soil microbial community of diversity index showed that the Shannon index of bacterial/fungal community under L was significantly higher than that of S, but the index of chao1, ACE and Simpson showed no significant difference between L and CK.?Under L, M and S treatments, the relative abundance of dominant bacteria genera Nocardioides, Pedobactor, Massilia and Promicromonospora were all higher than that of CK.?Under L treatment the genera of Earthbacter, Marseillae and Promicromonospora were significantly or extremely significantly higher than that of CK.?The relative abundance of dominant genera of Olpidium and Alternaria under L, M and S treatments was higher than that of CK, and the relative abundance of dominant genera Olpidium and Alternaria under S treatment was the highest (16.02%), which was significantly higher than that of other treatments, and the the relative abundance of Alternaria was highest under M treatment, which was significantly different from CK, L and S (P < 0.05) treatments.?Principal component analysis showed that bacterial community composition was similar between L and M, and fungal community composition was more similar between CK and L.?The results of Redundancy analysis showed that soil water content was negatively correlated with the bacterial communities of Nocardia, Marseillaria, norank_f__Saccharimonadaceae, Promicromonospora and Geobacter.?Soil water content, sucrase, urease and alkaline phosphatase activities were positively correlated with fungi of Mortierella and Acremonium, but negatively correlated with fungi of Gibberella, oleomycete and Cladosporium.?In conclusion, mild water stress (60% - 65% of field capacity) could significantly increase soil invertase activity and rhizosphere soil microbial diversity, and obvious change of soil microbial community structure to improve the adaptability of crops to drought. It was important for winter wheat to regulate growth against soil water stress during the key growth period. |
| Key words: Winter wheat; Water stress; Soil enzymatic activity; Soil microbial community structure and diversity |
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