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引用本文:韩阳,郝鲜俊,张又丹.不同磷水平下接种AM真菌对采煤塌陷区土壤GRSP量及玉米生长的影响1[J].灌溉排水学报,2017,36(8):.
HAN Yang,HAO Xianjun,ZHANG Youdan.不同磷水平下接种AM真菌对采煤塌陷区土壤GRSP量及玉米生长的影响1[J].灌溉排水学报,2017,36(8):.
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不同磷水平下接种AM真菌对采煤塌陷区土壤GRSP量及玉米生长的影响1
韩 阳, 郝鲜俊, 张又丹
山西农业大学 资源环境学院, 山西 太谷 030801
摘要:
煤炭大量开采造成地面塌陷,使土壤结构破坏,肥力下降,阻碍作物正常生长和对养分的吸收。丛枝菌根真菌(Arbuscular mycorrhizal fungi,AM真菌)在恢复退化的农林生态系统中具有重要的生理功能,尤其促进对土壤磷的吸收。通过盆栽试验,研究4个施磷水平(0、25、50 和100 mg/kg)下单接种摩西球囊霉(Glomus mosseae, G.m)、双接种幼套球囊霉(Glomus etunicatum, G.e)与摩西球囊霉对采煤塌陷区土壤球囊霉素相关土壤蛋白(GRSP)、玉米生长及地上部磷吸收的影响。结果表明,接种与磷水平及他们之间的交互作用对AM真菌丛枝丰度、株高、茎粗和易提取球囊霉素相关土壤蛋白(EE-GRSP)量具有显著影响(P<0.05);接种处理AM真菌丛枝丰度在0.29%~25.22%之间,同一磷水平双接种处理显著高于单接种处理(P<0.05)。而且GRSP量与AM真菌丛枝丰度表现出显著的二次曲线相关。相同磷水平下,总球囊霉素相关土壤蛋白(T-GRSP)和易提取球囊霉素相关土壤蛋白(EE-GRSP)量均呈双接种≥单接种>不接种,同一接种处理GRSP量随磷水平提高先升后降。虽然单接种和不接种处理在不同磷水平间对玉米生长的影响差异不显著,但是双接种处理显著提高了玉米株高、茎粗、地上部干质量及地上部吸磷量,表现为50、100 mg/kg磷水平显著高于0、25 mg/kg磷水平;相关性分析表明,AM真菌丛枝丰度与GRSP量、株高、茎粗、地上部磷量呈显著正相关。
关键词:  磷水平; AM真菌; 采煤塌陷区; 球囊霉素相关土壤蛋白; 玉米
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The Impact of AM Fungi and Phosphorus on Glomalin-related Soil Protein and Growth of Maizein a Coal-mining Induced Subsidized Area
HAN Yang, HAO Xianjun, ZHANG Youdan
College of Resources and Environment, Shanxi Agricultural University, Taigu 030801, China
Abstract:
Coal mining could result inland subsidence, leading to a deterioration in soil structures and decrease in soil fertility, hindering nutrient uptake and crop growth. Arbuscular mycorrhizal fungi (AMF) has special physiological functions in rehabilitating degraded agroforestry ecosystems and increasing crop growth, especially phosphorus (P) uptake. This paper presents the results of a greenhouse experiment in attempts to study the changes in glomalin-related soil protein (GRSP), maize growth and P uptake in a coal-mining induced subsided area underfour P application rates (0,25, 50 and 100 mg/kg) with the soil inoculated either by Glomus mosseae (single inoculation)or by Glomus etunicatum and Glomus mosseae (double inoculation). The results showed AMF inoculation, P addition and the interaction between AMF and P had a significant impact on AMF abundance, plant height and stem diameter, as well as the easy-to-extract glomalin-related soil protein (EE-GRSP) content (P<0.05). The AMF abundance was between 0.29% and 25.22%, with the abundance under double inoculation significantly higher than that under single inoculation (P<0.05) at the same P level. The relationship between AM abundance and GRSP content was found to be quadratic, and at the same P level the total glomalin-related soil protein (T-GRSP) and EE-GRSP were in the order of double inoculated ≥ single inoculated > non-inoculated. With the increase in P, the GRSP content increased first and then decreased with the AMF inoculation. Compared with non-inoculation, plant height, stem diameter, dry-weight of the shoot, as well as the P uptake remained almost unchanged under the single inoculation, while increased significantly under the double inoculation at the same P level. Also, under the four treatments with double inoculation, the plant height and P uptake at P additions of 50 and 100 mg/kg were significantly higher than that at the additions of 0 and 25 mg/kg. The AM abundance correlated positively with GRSP content, plant height, stem diameter and shoot P content.
Key words:  phosphorus level; arbuscular mycorrhizal fungi; coal mining subsidence area; glomalin-related soil protein; maize growth