| 引用本文: | 丁嘉宁,梁利宝,冯鹏艳,等.施肥对采煤塌陷复垦土壤理化性状和细菌群落结构影响的研究[J].灌溉排水学报,2020,39(12):64-70. |
| ,et al.施肥对采煤塌陷复垦土壤理化性状和细菌群落结构影响的研究[J].灌溉排水学报,2020,39(12):64-70. |
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| 摘要: |
| 【目的】探讨施肥对采煤塌陷复垦土壤理化性状和细菌群落结构的影响，为复垦土壤培肥提供参考。【方法】以山西省晋城市采煤塌陷区复垦土壤为对象，设置不施肥（对照CK）、有机肥（M）、无机肥（W）、50%有机+50%无机肥（WM）4个不同施肥处理。采用Illumina高通量测序平台研究了不同施肥对细菌群落结构的影响，并分析了细菌群落结构变化与土壤理化性质的相关性。【结果】与CK相比，①M、WM处理显著（p<0.05）增加了1~2 mm团聚体比例，降低了0.053~0.25 mm、<0.053 mm的团聚体比例。②M处理显著（p<0.05）提升土壤有机质量、全氮量、全磷量，W、WM处理显著（p<0.05）增加了速效养分。③M处理显著（p<0.05）增加了磷酸酶、转化酶活性，W处理显著（p<0.05）增加了脲酶活性。④M处理和WM处理显著（p<0.05）增加了变形菌门的丰度，M处理和W处理则显著（p<0.05）增加了放线菌门的丰度。⑤冗余分析表明：全磷、有机质、碱解氮和体积质量是造成土壤细菌群落丰度差异的主要因子，对不同施肥处理土壤细菌群落丰度的影响大小排序为：M处理>W处理>WM处理>CK。【结论】施肥不同程度地改善了复垦土壤理化性状，全磷、有机质、碱解氮和体积质量是影响土壤微生物群落结构的主要因子。 |
| 关键词: 施肥；复垦土壤；土壤理化性状；细菌群落；环境因子 |
| DOI：10.13522/j.cnki.ggps.2020077 |
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| Effects of Fertilization on Physical and Chemical Properties and Bacterial Community of Reclaimed Soil from Coal Mining Subsidence |
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DING Jianing, LIANG Libao*, FENG Pengyan, XU Jianmin
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College of Resources and Environment, Shanxi Agricultural University, Taigu 030801, China
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| Abstract: |
| 【Objective】Research the effects of fertilization on the physical and chemical properties and bacterial community structure of reclaimed soil after coal mining collapse, to provide reference for soil fertility cultivation.【Method】Taking the reclaimed soil of mining subsidence area of Jincheng, Shanxi Province as the object area, four different fertilization treatments were set up, namely respectively no fertilization（CK）, organic fertilizer（M）, inorganic fertilizer（W）, 50% organic + 50% inorganic fertilizer（WM）. Illumina Miseq high-throughput sequencing platform was used to study the effects of different fertilizers on bacterial community structure, and the correlation between the change of bacterial community structure and soil physical and chemical properties was analyzed. 【Result】Compared with CK, ①Treatment M and WM significantly（p<0.05）increased the aggregate ratio of 1~2 mm and decreased the aggregate ratio of 0.053~0.25 mm and <0.053 mm. ②Treatment M significantly increased soil organic matter, total nitrogen, total phosphorus, while treatment W and WM significantly increased available nutrients. ③Treatment M increased the activity of phosphatase and invertase, while treatment W increased the activity of urease. ④Treatment M and WM increased the abundance of Proteus significantly, while treatment M and W increased the abundance of actinomycetes significantly. ⑤The results of redundancy analysis showed that total phosphorus, organic matter, alkali-hydrolyzed nitrogen and volume mass were the main reasons for the differences of bacterial community abundance. The order of the influence on the bacterial community abundance of different fertilization treatments was: M>W>WM>CK.【Conclusion】Fertilization improved the physical and chemical properties of reclaimed soil in different degrees. Total phosphorus, organic matter, alkali-hydrolyzed nitrogen and volume mass were the significant factors affecting the structure of soil microbial community. |
| Key words: fertilization; reclaimed soil; soil physical and chemical properties; bacterial community; environmental factors |
