| 引用本文: | 孙文君,江晓慧,付媛媛,等.盐分胁迫对棉花幼苗叶片叶绿素荧光参数的影响[J].灌溉排水学报,2021,(7):23-28. |
| SUN Wenjun,JIANG Xiaohui,FU Yuanyuan,et al.盐分胁迫对棉花幼苗叶片叶绿素荧光参数的影响[J].灌溉排水学报,2021,(7):23-28. |
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| 摘要: |
| 【目的】研究盐分胁迫对棉花幼苗叶片叶绿素荧光特性的影响，为盐分胁迫抑制棉花幼苗生理过程提供参考。【方法】在人工气候室内以“新陆中37”为供试材料，分析了50、100、150 mmol/L和200 mmol/L共4个盐分处理以及浇灌Hoagland营养液作为对照（CK）下棉花叶片叶绿素荧光参数的变化特征。【结果】50 mmol/L盐分处理的棉花叶片叶绿素相对量（SPAD）、最大量子效率(Fv/Fm)、实际量子效率(Y(II))和光化学淬灭((qP))等参数值与CK接近或略低于CK，最大荧光(Fm)略高于CK，但100~200 mmol/L盐分处理的SPAD、Fv/Fm等参数显著降低，(Y(II))在200 mmol/L盐分处理时呈下降趋势；最小荧光(Fo)在各盐分处理间大幅度下降，非光化学淬灭参数qN则差异不显著，而100~200 mmol/L盐分处理的过剩光能((1-qP)/NPQ)和非光化学淬灭参数NPQ则较CK分别显著提高21.5%、25.7%、43.3%与6.4%、20.8%、23.8%，但50 mmol/L盐分处理与CK间的差异不显著。【结论】棉花在低物质的量浓度50~100 mmol/L范围里通过自身的调节机制能够避免光反应系统受到损伤，而当盐分物质的量浓度达到150 mmol/L时，引发光合电子传递受阻，棉花叶片受到光抑制，导致叶片的光能利用效率显著降低，最终导致植物生长受到抑制。 |
| 关键词: 棉花幼苗；盐胁迫；荧光参数；叶绿素相对量 |
| DOI：10.13522/j.cnki.ggps.2020480 |
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| The Effects of Salt Stress on Chlorophyll Fluorescence of Cotton Seedling Leaves |
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SUN Wenjun, JIANG Xiaohui, FU Yuanyuan, SHEN Xiaojun, GAO Yang, WANG Xingpeng
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1. College of Water Resource and Architecture Engineering, Tarim University, Alaer 843300, China;
2. College of Hydraulic and Civil Engineering, Shandong Agricultural University, Tai’an 271018, China;
3. Key Laboratory of Crop Water Demand and Regulation, Ministry of Agriculture and Rural Affairs/
Institute of Field Irrigation, Chinese Academy of Agricultural Sciences, Xinxiang 453002, China
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| Abstract: |
| 【Objective】 To study the effects of salt stress on chlorophyll fluorescence characteristics of cotton seedling leaves, and it provides reference for salt stress to inhibit the physiological process of cotton seedlings. 【Method】 Using “Xinluzhong 37” as the experimental material in the artificial climate chamber, the changes of chlorophyll fluorescence parameters in cotton leaves were analyzed with four salt concentrations of 50, 100, 150 mmol/L and 200 mmol/L and Hoagland nutrient solution as the control treatment (CK).【Result】 Results showed that the relative chlorophyll content (SPAD), maximum quantum efficiency (Fv/Fm), actual quantum efficiency (Y(II)) and photochemical quenching (qP) of cotton leaves in the treatment of 50 mmol/L were close to or lower than those values in CK, The maximum fluorescence (Fm) was slightly higher than that of CK, but the SPAD Fv/Fm Y(II) and other parameters were significantly decreased under 100~200 mmol/L salt treatment, and (Y(II)) showed a decreasing trend under 200 mmol/L salt treatment. However, the excess light energy ((1-qP)/NPQ) and non-photochemical quenching parameter NPQ of 100~200 mmol/L high salt treatment were significantly increased by 21.5%, 25.7%, 43.3% and 6.4%, 20.8% and 23.8%, respectively, compared with the control treatment. However, there was no significant difference between 50 mmol/L low salt concentration treatment and the control treatment. 【Conclusion】 Cotton can avoid the damage of light reaction system through its own regulation mechanism in the low salt concentration of 50~100 mmol/L. When the salt concentration reaches 150 mmol/L, the light emission and electron transfer of cotton leaves are blocked, resulting in the significant decrease of light energy utilization efficiency of cotton leaves, and finally limiting the plant growth. |
| Key words: cotton seedling; salt stress; fluorescence parameters; relative chlorophyll content |
