| 引用本文: | 邢玉美,万云婷,刘延龙,等.宛氏拟青霉提取物对淹水胁迫下玉米叶片叶绿素荧光动力学参数的影响[J].灌溉排水学报,2021,(10):52-57. |
| XING Yumei,WAN Yunting,LIU Yanlong,et al.宛氏拟青霉提取物对淹水胁迫下玉米叶片叶绿素荧光动力学参数的影响[J].灌溉排水学报,2021,(10):52-57. |
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| 摘要: |
| 【目的】探究宛氏拟青霉提取物（PVE）提高玉米幼苗对淹水胁迫适应能力的光合生理机制。【方法】通过人工模拟淹水胁迫环境，研究不同程度淹水胁迫后叶面喷施清水和PVE对玉米幼苗生长和叶片快速叶绿素荧光诱导动力学曲线（OJIP）的影响。【结果】淹水胁迫显著抑制了玉米幼苗生长，降低了叶片的净光合速率。长期淹水胁迫（＞4 d）导致玉米幼苗叶片OJIP曲线发生明显变化，增加了在O相处的初始荧光强度（Fo），但降低了叶片I相（30 ms）和P相（最大荧光）荧光值。与正常水分处理相比，淹水胁迫减少了单位面积内反应中心的数量（RC/CSm），抑制了QA向QB的传递过程和电子传递能力（Ψo和δRo）。与叶面喷施清水处理相比，解除短期淹水胁迫（≤2 d）后叶面喷施PVE缓解了单位叶面积吸收（ABS/CSm）、捕获（TRo/CSm）和传递（ETo/CSm）的光能的降低，使叶片维持相对较高的单位叶面积热耗散能力（DIo/CSm），降低了激发能的产生，减少过剩激发能对PSⅡ反应中心的伤害，但长期淹水胁迫（＞4 d）下，叶面喷施PVE并不能完全消除淹水胁迫对叶片光合特性和OJIP曲线的影响。【结论】叶面喷施PVE能够增强玉米幼苗抵御短期淹水胁迫的能力，有助于解除淹水胁迫后的迅速恢复生长。 |
| 关键词: 玉米；淹水胁迫；宛氏拟青霉提取物；叶绿素荧光 |
| DOI：10.13522/j.cnki.ggps.2021108 |
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| Effects of Extracts from Paecilomyces Variotii on Chlorophyll Fluorescence Transient in Leaves of Maize under Waterlogging Stress |
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XING Yumei, WAN Yunting, LIU Yanlong, WANG Pengfei, FAN Zhen, ZHAO Youxin, TIAN Xiaofei
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(School of Environment and Planning, Liaocheng University, Liaocheng 252059, China)
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| Abstract: |
| 【Background and objective】Waterlogging is an abiotic stress occurring frequently during maize growth season, especially in its initial seedling stage. The crude extract of endophyte Paecilomyces variotii is a bio-stimulant widely used in China to promote plant growth and improve its resistance against waterlogging. We extracted Paecilomyces variotii (PVE) from the endophytic fungus in a wild sea buckthorn, which is not only able to protect crops from pathogens but also promotes crop growth. However, the underlying mechanisms of how PVE enhances the tolerance of maize against waterlogging is obscure. The aim of this paper is to fill this knowledge gap.【Method】We used maize as the model plant and conducted the experiment in laboratory. Maize seedings treated with PVE were subjected to waterlogging, with seedlings not treated with PVE taken as control. For each treatment, we measured the change in chlorophyll fluorescence transient curve (OJIP) of the seedings. 【Result】Waterlogging hindered growth of the maize seedling and reduced its net photosynthetic rate (Pn) as a result. Waterlogging lasting more than four days changed the OJIP curve by reducing the active reaction centre per unit area, and altered the ability of electronic transmission from QA to QB (Ψo and δRo). Treating the seedlings with PVE alleviated the waterlogging-induced decrease in specific energy fluxes per unit area for absorption (AB/CSm), trapping (TRo/CSm), and electron transport (ETo/CSm), while in the meantime maintaining a relatively high heat dissipation capacity (DIo/CSm).【Conclusion】Spraying PVE over maize seedings enhanced their tolerance to waterlogging stress and helped their quick recovery after the waterlogging receded. |
| Key words: maize; waterlogging stress; extracts from Paecilomyces variotii; chlorophyll fluorescence transient |
