| 引用本文: | 田雨雨,李东伟,曲子良,等.生物炭协同暗管排水对滨海盐碱土壤微生物群落的影响[J].灌溉排水学报,2024,43(4):82-89. |
| TIAN Yuyu,LI Dongwei,QU Ziliang,et al.生物炭协同暗管排水对滨海盐碱土壤微生物群落的影响[J].灌溉排水学报,2024,43(4):82-89. |
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| 摘要: |
| 【目的】了解滨海地区盐渍化环境下生物炭协同暗管排水作用与土壤微生物群落的关系，对于减轻土壤盐分过高对土壤生化功能的不利影响至关重要。【方法】本文以黄河三角洲滨海盐碱农田为研究对象，研究单独暗管排水条件下不同暗管间距10 m（S10）、20 m（S20）、30 m（S30）以及生物炭协同暗管排水条件下不同暗管间距10 m（B-S10）、20 m（B-S20)、30 m（B-S30）对土壤理化性质和土壤微生物群落结构的影响，并阐明生物炭与暗管排水协同作用下微生物群落结构变化的主要驱动因素。【结果】生物炭协同暗管排水改变了滨海盐碱农田的土壤理化性质，且生物炭与暗管间距对含盐量、硝态氮、有机质、土壤全磷的交互作用显著。AccuITSTM和Accu16STM绝对定量测序结果显示，暗管间距及暗管间距和生物炭的交互作用对伞菌纲和Acidobacteria_Gp4有显著影响。冗余分析、皮尔逊相关性分析结果表明，对作物生长有利的伞菌纲和被孢霉的绝对丰度与土壤含盐量和Na+显著负相关，与有机质和硝态氮显著正相关。生物炭协同暗管排水作用下，Na+量是影响土壤细菌和真菌的关键因素。【结论】B-S10既可以改善土壤的养分状况，又可以改善土壤微生物群落结构，本研究可为解决盐碱土地问题以及提高土壤质量和农业可持续性提供了有益的理论参考。 |
| 关键词: 暗管排水；生物炭；滨海盐碱地；微生物群落组成；绝对定量 |
| DOI：10.13522/j.cnki.ggps.2023527 |
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| Effect of biochar amendment and subsurface drainage on microbial communities in saline coastal soils |
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TIAN Yuyu, LI Dongwei, QU Ziliang, JING Rui, ZHAO Qingqing, ZHOU Xinguo
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1. Farmland Irrigation Research Institute, Chinese Academy of Agricultural Sciences, Xinxiang 453002, China;
2. Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100080, China;
3. Dandong Hydrographic Bureau of Liaoning Province, Dandong 118000, China
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| Abstract: |
| 【Background】Soil microorganisms underpin soil functions, and its community is influenced by a multitude of abiotic and biotic factors. The objective of this paper is to study the combined effect of biochar amendment and subsurface drainage on microbial communities in salinized soil in coastal ecosystems.【Method】 The experiment was conducted in a field at the Yellow River delta, with the subsurface drains spaced by10 m (S10), 20 m (S20), and 30 m (S30), respectively. For each drain spacing, there was a biochar amendment treatment and non-biochar treatment. During the experiment, we measured the changes in soil physicochemical properties and microbial community structure. And the main driving factors for the changes in microbial community structure under synergistic effect of biochar and subsurface drainage were elucidated.【Result】Biochar amendment and subsurface drainage altered soil physicochemical properties. Biochar amendment and drain spacing were interactive having a significant impact on salinity, nitrate nitrogen, organic matter and total phosphorus in the soil. The sequencing of AccuITSTM and Accu16STM showed that drain spacing working separately or in combination with biochar amendment had a significant effect on Agaricomycetes and Acidobacteria_Gp4, albeit the underlying mechanisms are elusive. Redundancy analysis and Pearson correlation analysis showed that the absolute abundance of Agaricomycetes and Mortierellomycetes, beneficial for crop growth, were negatively correlated with soil salinity and sodium ions while positively correlated with organic matter and nitrate nitrogen, both at significant levels. Sodium ion content was the key factor affecting soil bacteria and fungi in all treatments we compared.【Conclusion】Amending the salinized soil by biochar coupled with subsurface drainage with the drains spaced 10 m apart worked best for improving soil nutrient and soil microbial community structure. It can be used as an improved technique for soil reclamation and agriculture production in salinized soils in coastal regions. |
| Key words: subsurface drainage; biochar; coastal saline soils; microbial community composition; absolute quantification |
