| 引用本文: | 程亚南,李延峰,王 菲,等.黄淮海平原潮土酶活性和土壤呼吸对
阿特拉津的短期响应[J].灌溉排水学报,2025,44(10):111-119. |
| CHENG Yanan,LI Yanfeng,WANG Fei,et al.黄淮海平原潮土酶活性和土壤呼吸对
阿特拉津的短期响应[J].灌溉排水学报,2025,44(10):111-119. |
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| 摘要: |
| 【目的】评估阿特拉津对潮土的生态效应，筛选可表征其污染强度的生物标志物。【方法】基于培养试验，设置0（CK）、0.6（T1）、2.5（T2）、10（T3）、50（T4）、100（T5）mg/kg 6个阿特拉津浓度，研究其对土壤酶活性和土壤呼吸的影响，并通过IBRv2指数评估其综合毒性。【结果】阿特拉津显著激活了土壤脲酶活性，且存在显著的剂量-效应关系。蔗糖酶活性在第14 d时被显著抑制，在28 d后恢复至CK水平。培养前期，高浓度阿特拉津显著抑制了淀粉酶活性，且抑制程度与浓度呈显著正相关。土壤碱性磷酸酶在培养7 d时对阿特拉津胁迫没有响应，第14~第21 d时其活性被激活，之后被显著抑制。阿特拉津显著激活了土壤过氧化氢酶活性（T1处理除外），且激活程度与浓度成正比。培养28 d后，不同浓度的阿特拉津均显著促进了土壤呼吸，且浓度越高呼吸强度越高。IBRv2结果表明，阿特拉津浓度越高，其对土壤产生的综合毒性越大。【结论】土壤脲酶、淀粉酶和过氧化氢酶对阿特拉津胁迫响应十分迅速，其活性与阿特拉津浓度间存在显著的剂量-效应关系，可作为表征阿特拉津污染程度的生物标志物。 |
| 关键词: 阿特拉津；酶活性；土壤呼吸；IBRv2 |
| DOI：10.13522/j.cnki.ggps.2024334 |
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| Short-term response of soil enzymatic activities and respiration to atrazine in fluvio-aquic soil in the Huang-Huai-Hai Plain |
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CHENG Yanan, LI Yanfeng, WANG Fei, LI Yonggang, REN Xiujuan
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School of Plant Protection and Environment, Henan Institute of Science and Technology, Xinxiang 453003, China
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| Abstract: |
| 【Objective】Atrazine is a widely used herbicide whose ecological impacts on fluvio-aquic soils remain insufficiently understood. This study aims to evaluate the effects of atrazine on soil biogeochemical properties and to identify sensitive biomarkers capable of indicating atrazine pollution level.【Method】A controlled culture experiment was conducted using six atrazine concentrations 0 (CK), 0.6 (T1), 2.5 (T2), 10 (T3), 50 (T4), and 100 mg/kg (T5). The changes in soil enzyme activities (urease, sucrase, amylase, alkaline phosphatase, and catalase) were measured daily from the onset of the experiment to elucidate the temporal variations in their response to atrazine application. The IBRv2 index was used to evaluate the comprehensive toxicity of atrazine to soil biogeochemical processes.【Result】Atrazine significantly stimulated soil urease activity, but the effect varied with atrazine concentration. Soil sucrase activity was significantly inhibited on day 14 after atrazine application but returned to CK levels after 28 days. Increasing atrazine concentrations strongly inhibited soil amylase activity during the early incubation period, with the inhibition positively correlated with atrazine concentration. The activity of alkaline phosphatase did not show a noticeable change in the first 7 days after atrazine application, but became significant between day 14 and day 21 before being inhibited. Atrazine significantly stimulated catalase activity except in T1 treatment, with the activation increasing with atrazine concentration. Soil respiration started to increase significantly 28 days after atrazine application, and it was positively correlated with atrazine concentration. IBRv2 analysis showed that increasing atrazine concentrations resulted in an increase in soil toxicity.【Conclusion】The activity of soil urease, amylase and catalase responded rapidly and sensitively to atrazine application, with the changes in activity level depending on atrazine concentration. These enzymes can serve as biomarkers to assess the impact of atrazine on biogeochemical processes in fluvio-aquic soils. |
| Key words: atrazine; enzyme activity; soil respiration; IBRv2 |
