| 摘要: |
| 【目的】明确草莓叶片光合作用对外源有机硒的响应。【方法】采用盆栽试验叶面喷施法，设置5种硒代蛋氨酸（SeMet）添加量处理：CK（0 mg/L）、T1处理（10 mg/L）、T2处理（30 mg/L）、T3处理（60 mg/L）、T4处理（90 mg/L），研究了外源SeMet不同添加量对甜查理草莓大果期、转色期以及成熟期的叶片光合色素量、光合参数与叶绿素荧光参数、植株总生物量及果实糖度的影响。【结果】外源SeMet显著提高各时期草莓叶片叶绿素a（Chla）、叶绿素b（Chlb）、叶绿素总量（Chla+b）、类胡萝卜素（Car）量以及转色期Chla/b。与CK相比，T3处理各时期Chla量分别增加47.1%、75.7%、54.2%，Chlb量分别增加22.5%、37.1%、47.9%，Chla+b量分别增加30.9%、59.9%、50.4%，Car量分别增加31.9%、24.1%、33.7%。外源SeMet还显著提高了叶片光合速率（Pn）、气孔导度（Gs）、蒸腾速率（Tr）、水分利用效率（WUE）。与CK相比，T3处理使Pn分别增加44.1%、53.8%、82.5%；Gs分别增加29.7%、39.7%、90%，Tr分别增加21.4%、34.4%、44%，WUE分别增加18.7%、14.5%、26.7%。同时，SeMet对叶绿素荧光参数也产生了显著影响。与CK相比，T3处理使各时期Y（II）分别增加了22.5%、16%、20%，Fv/Fm分别增加1.34%、21.1%、9.4%，Fv'/Fm'分别增加28.9%、9.5%、20.9%，ETR分别增加14.6%、12.7%、22.9%，qP分别增加22.6%、19.4%、28.4%，NPQ分别降低35.6%、27.7%、51.6%。此外，SeMet还显著提高了各时期草莓植株总生物量及果实糖度，且均以T3处理的效果最为显著。【结论】60 mg/L外源 SeMet能够改善草莓叶片的光合能力及水分利用效率，同时提高干物质积累及果实糖量，可应用于生产中以优化草莓栽培措施，达到提升草莓果实品质的目的。 |
| 关键词: 草莓；硒代蛋氨酸；水分利用效率；光合作用；糖度；植株总生物量 |
| DOI：10.13522/j.cnki.ggps.2020160 |
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| The Effects of Selenomethionine Application on Photosynthesis and Chlorophyll fluorescence of Strawberry |
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GAO Shang, CAI Liping, SHAN Changjuan
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1. Henan Institute of Science and Technology, Xinxiang 453003, China; 2. Luohe Vocational College of Food, Luohe 462000, China
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| Abstract: |
| :【Objective】The objective of this paper is to unravel the response of photosynthesis of strawberry leaves to application of exogenous organic selenium at different concentrations.【Method】The experiment was conducted in pots using the variety of “sweet Charlie” as the model plant. Elenomethionine (SeMet) was applied to the pots at a concentration gradient varying from 0 (CK) to 90 mg/L. In each treatment, we measured the photosynthetic pigment content, photosynthetic parameters, chlorophyll fluorescence parameters, total plant biomass and sugar content at the fruit growing stage, colour-changing stage and mature stage, respectively.【Result】Applying SeMEt significantly increased the contents of Chl-a, Chl-b and Car, regardless of the growth stages, especially when SeMet concentration was 60 mg/L. Compared with CK, applying 60 mg/L of SeMet increased Chla content by 47.1%, 75.7%, 54.2%; Chlb content by 22.5%, 37.1%, 47.9%, at fruit-growing, colour-changing and mature stage, respectively. Applying SeMet also significantly increased Pn, Gs, Tr and WUE of the leaves, especially when applied at 60 mg/L, which increased Pn by 44.1%, 53.8%, 82.5%; Gs by 29.7%, 39.7%, 90%; Tr by 21.4%, 34.4%, 44%; and WUE by 18.7%, 14.5%, 26.7%, respectively, at the three growth stages. SeMet application also had significant effects on chlorophyll fluorescence parameters. On average, applying SeMet at 60 mg/L was most significant. Compared with CK, applying 60 mg/L of SeMet increased Y(II) by 22.5%, 16%, 20%; Fv/Fm by 1.34%, 21.1%, 9.4%; Fv'/Fm' by 28.9%, 9.5%, 20.9%; ETR by 14.6%, 12.7%, 22.9%. qP by 22.6%, 19.4% , 28.4%; and NPQ by 35.6%, 27.7%, 51.6%, respectively, at the three growth stages. In addition, applying SeMet also increased the total biomass and sugar content, especially at 60 mg/L.【Conclusion】Applying 60 mg/L of SeMet improved leaf photosynthesis, water use efficiency, as well as plant dry biomass accumulation and fruit sugar content. |
| Key words: strawberry; selenomonine; water use efficiency; photosynthesis; sugar content; total plant biomass |
