| 引用本文: | 李焕弟,缴锡云,李 江,等.基于MICP及EICP技术的土壤固化试验研究[J].灌溉排水学报,2021,(7):59-65. |
| LI Huandi, JIAO Xiyun, LI Jiang, SU Mingxiao, WU Shuyu, LIU Yong,LI Huandi, JIAO Xiyun, LI Jiang, SU Mingxiao, WU Shuyu, LIU Yong,LI Huandi, JIAO Xiyun, LI Jiang, SU Mingxiao, WU Shuyu, LIU Yong,et al.基于MICP及EICP技术的土壤固化试验研究[J].灌溉排水学报,2021,(7):59-65. |
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| 基于MICP及EICP技术的土壤固化试验研究 |
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李焕弟，缴锡云，李 江，苏明晓，巫纾予，刘 永
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1.河海大学 农业科学与工程学院，南京 210098；2.水文水资源与水利工程国家重点实验室，
南京 210098；3.河海大学 水安全与水科学协同创新中心，南京 210098；
4.清华大学 水沙科学与水利水电工程国家重点实验室, 北京 100084
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| 摘要: |
| 【目的】针对传统土质边坡固化方法操作复杂、维修维护困难问题，开展基于微生物诱导碳酸钙沉积（MICP）及脲酶诱导碳酸钙沉积（EICP）技术的土壤固化研究，为农田土质排水沟边坡生态防护提供一定的理论指导。【方法】基于MICP及EICP技术的土壤固化研究，使用巴氏芽孢杆菌和脲酶进行土壤固化试验，设置巴氏芽孢杆菌（菌组）、脲酶（脲酶组）处理，通过溶液试验，对比研究巴氏芽孢杆菌菌液、脲酶溶液分别与固结液（尿素/氯化钙混合液）以不同配比（1∶3、2∶3、1∶1、4∶3、5∶3）混合下的碳酸钙生成量，并进行土壤多轮次喷洒试验，分析不同固化轮次下土壤表面形态、碳酸钙量和微观结构特征，探讨巴氏芽孢杆菌、脲酶对土壤的固化效果。【结果】巴氏芽孢杆菌、脲酶作为MICP和EICP过程的催化剂水解尿素，然后与氯化钙生成碳酸钙晶体，不同催化剂与固结液的最佳配比为菌组2∶3、脲酶组5∶3，其碳酸钙产率分别为86.7%、84.1%；2个处理以2∶3配比对土壤进行多轮次喷洒，生成的碳酸钙可有效修复土壤表层裂缝，菌组碳酸钙量比脲酶组高，但其差距在后期逐渐减小；巴氏芽孢杆菌和脲酶诱导产生的碳酸钙晶体形态不同。【结论】巴氏芽孢杆菌、脲酶诱导沉积的碳酸钙晶体填充在土颗粒之间，可达到良好的土壤固化效果，本研究条件下，巴氏芽孢杆菌处理效果比脲酶处理好。 |
| 关键词: 巴氏芽孢杆菌；脲酶；土壤固化；碳酸钙量；微观结构 |
| DOI：10.13522/j.cnki.ggps.2020608 |
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| Using Microbe-induced Calcite Precipitation and Enzyme-induced Carbonate Precipitation to Cement Slopes of Earth Ditches |
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LI Huandi, JIAO Xiyun, LI Jiang, SU Mingxiao, WU Shuyu, LIU Yong
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1. College of Agricultural Science and Engineering, Hohai University, Nanjing 210098, China;
2. State Key Laboratory of Hydrology-water Resources and Hydraulic Engineering, Hohai University,
Nanjing 210098, China; 3. Collaborative Innovation Center for Water Security and Water Science,
Hohai University, Nanjing 210098, China; 4. State Key Laboratory of Hydroscience and Engineering,
Department of Hydraulic Engineering, Tsinghua University, Beijing 100084, China
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| Abstract: |
| 【Background】Open earth ditches are a common drainage facility to keep groundwater table below a critical depth to prevent salt accumulation, but they are prone to collapse. Traditional methods to consolidate earth trench are expensive and difficult to maintain, and biotechnological technologies such as microbe-induced calcium carbonate precipitation have become a promising alternative due to their easy operation and less impact on the environments.【Objective】The purpose of this paper is to study the feasibility and efficacy of microbe-induced calcite precipitation (MICP) and enzyme-induced carbonate precipitation (EICP) to consolidate and stabilize earth ditches.【Method】We used Sporosarcina pasteurii and urease, respectively, in this paper, with each mixed with urea-calcium chloride solution at various volumetric ratios ranging from 1∶3 to 5∶3. The mixed solution was sprayed over the soil surface. The changes in soil surface morphology, calcite content and soil microstructure in each treatment were measured periodically until they stabilized to an equilibrium.【Result】Both sporosarcina pasteurii and urease can be used as a catalyzer to hydrolyze urea and form calcium carbonate crystals from the calcium chloride solution. For sporosarcina pasteurii, the best result was when its ratio to the calcium chloride solution was 2∶3, while for the urease solution, the result was optimal when their volumetric ratio was 5∶3, with the highest calcium yield of the former and the latter being 86.7%, 84.1%, respectively. Spraying the soil surface with either mixture several times can fill the cracks in the soil although the calcite produced by MICP was more than that generated by EICP. However, the difference between the two waned as time elapsed; the calcium carbonate crystals formed in MICP differed from that in EICP. 【Conclusion】 Both Sporosarcina pasteurii and urease can catalyze the hydrolysis of urea to form calcite, which can fill the pores to consolidate the soil. Our experimental results showed MICP is more effective than EICP. |
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