| 摘要: |
| 【目的】研究不同干旱胁迫处理对马铃薯“冀张薯12”代谢物及代谢通路的影响。【方法】选用“冀张薯12”为研究对象，采用盆栽法，设中度干旱处理（35%~40%土壤相对含水率，T1）、重度干旱处理（15%~20%土壤相对含水率，T2）和对照处理（保持土壤相对含水率为65%~70%并持续14 d，CK），研究不同干旱胁迫处理对马铃薯体内代谢物含量及代谢途径的影响。【结果】T1处理与CK相比，有39个代谢物量发生显著变化，其中上调的有20个，下调的有19个，涉及29种代谢途径；T2处理与CK相比，有158个代谢物量发生显著变化，其中上调的有67个，下调的有91个，涉及61种代谢途径。T2处理与T1处理相比，有37个代谢物量发生显著变化，其中上调的有26个，下调的有11个，涉及47种代谢途径。T1、T2处理分别与CK相比时，在各自差异显著的前20个代谢物中，均上调的有7个，包括：薯蓣皂苷元-3-O-葡萄糖基（1→4）鼠李糖基（1→4）鼠李糖基（1→2）葡萄糖苷、德尔托宁、原薯蓣皂苷、3-O-葡萄糖基-（1→2）-木糖基-（1→3）]-葡萄糖基-（1→4）-半乳糖苷-薯蓣皂苷元、5（6）-烯-螺旋甾-3-醇-3-O-葡萄糖基-（1→4）-[鼠李糖基-（1→2）]-半乳糖苷、5,6-二氢茄解碱、假原薯蓣皂苷；均下调的有3个，包括：N-（4'-O-糖基）-阿魏酰精胺、桢楠内酯D、松萝酸。同时，与CK相比，T1处理下代谢物富集最多的代谢途径为苯丙烷类生物合成、氨基酸的生物合成；T2处理下代谢物富集最多的代谢途径为亚油酸代谢、α-亚麻酸代谢。【结论】马铃薯遭受不同干旱胁迫后，其体内差异代谢物的数量、种类及涉及的代谢通路均存在差异，且会通过启动不同的代谢途径应对胁迫以实现自我保护。 |
| 关键词: 马铃薯；干旱胁迫；代谢物；代谢通路 |
| DOI：10.13522/j.cnki.ggps.2025267 |
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| 基金项目: |
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| Metabolomic changes in potato (Solanum tuberosum L.) under different drought stress levels |
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LIU Yaqi, SU Riguga, LIU Sujun
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1. College of Life Science and Technology, Inner Mongolia Normal University, Hohhot 010022, China;
2. Key Laboratory of Biodiversity Conservation and Sustainable Utilization in Mongolian Plateau for College and
University of Inner Mongolia Autonomous Region, Hohhot 010022, China
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| Abstract: |
| 【Objective】Drought is one of the major abiotic stresses limiting potato growth and yield in many regions. Understanding metabolic responses of potato to drought is essential for elucidating the mechanisms underlying its adaptation to drought and breeding drought-resistant cultivar, but it remains elusive. This paper aims to fill this knowledge gap.【Method】The experiment was conducted in pots using the Jizhangshu 12 variety as the model plant. There were three irrigation treatments by controlling soil moisture in the root zone at 35%-40% (T1), 15%-20% (T2) of the field capacity. The control was maintaining the soil moisture at 65%-70% of the field capacity for 14 days (CK). During the experiment, we measured various metabolite profiles and the associated metabolic pathways in the different treatments.【Result】In T1, there were 39 metabolites that showed significant differences from those in CK, including 20 upregulated and 19 downregulated metabolites, involved in 29 metabolic pathways. In T2, there were 158 metabolites that differed significantly from those in CK, including 67 upregulated and 91 downregulated, involved in 61 metabolic pathways. There were 37 metabolites that differed significantly between T1 and T2, including 26 upregulated and 11 downregulated, involved in 47 metabolic pathways. Among the top 20 metabolites in T1 and T2, which differed from those in CK, seven were upregulated: diosgenin-3-O-glucosyl-(1→4)-rhamnosyl-(1→4)-rhamnosyl-(1→2)-glucoside, deltonin, protodioscin, 3-O-glucosyl- (1→2)-xylosyl-(1→3)-glucosyl-(1→4)-galactoside-diosgenin, 5(6)-ene-spirost-3-ol-3-O-glucosyl-(1→4)-[rhamnosyl- (1→2)]-galactoside, 5,6- dihydrosolanidine, and pseudoprotodioscin; three were downregulated: N-(4′-O-glycosyl)- feruloyl spermidine, machilin D, and usnic acid. In T1, differentially accumulated metabolites were mainly enriched in 29 pathways, including metabolic pathways, biosynthesis of secondary metabolites, phenylpropanoid biosynthesis, amino acid biosynthesis, and flavonoid biosynthesis, while in T2, they were significantly enriched in 47 pathways, primarily metabolic pathways, biosynthesis of secondary metabolites, biosynthesis of cofactors, amino acid biosynthesis, and 2-oxocarboxylic acid metabolism.【Conclusion】Drought significantly altered metabolite composition and metabolic pathways in potato plants, especially under severe drought stress. Steroidal saponins and flavonoids were identified as potential positive biomarkers associated with drought resistance, whereas phenolic amides and sesquiterpene lactones showed negative responses to drought stress. These findings suggest that potatoes activate multiple metabolic pathways to mitigate drought effects and maintain physiological stability, providing insight into the metabolic mechanisms underlying drought tolerance. |
| Key words: potato; drought stress; metabolites; metabolic pathway |
