| 引用本文: | 张旭斌,董军林,邱小琮,等.太阳山湿地不同季节水体细菌群落结构差异及影响因素分析[J].灌溉排水学报,2026,45(10):98-106. |
| Zhang Xubin,Dong Junlin,Qiu Xiaocong,et al.太阳山湿地不同季节水体细菌群落结构差异及影响因素分析[J].灌溉排水学报,2026,45(10):98-106. |
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| 摘要: |
| 【目的】探究干旱区典型湿地水体细菌群落时空演替特征及其环境驱动机制。【方法】以宁夏太阳山湿地为对象，结合水环境理化指标分析与16S rRNA高通量测序技术，系统分析4、7月与10月水体细菌群落的结构差异及环境影响因素。【结果】①太阳山湿地水环境因子呈显著季节异质性，7月氨氮（NH3-N）较高；10月总磷（TP）和氟离子（F-）较高，4月氟离子亦维持较高水平。②水体细菌多样性表现为夏季显著高于春季和秋季（P＜0.05），“温度-营养盐”耦合机制维持了7月较高的物种丰富度，而4、10月低温与高氟化学胁迫可能增强环境筛选压力，并与细菌群落多样性降低相关。③3个季节中变形菌门、拟杆菌门和放线菌门均为水体细菌优势类群。此外，局部样点检测到脱硫微菌属（Desulfomicrobium）与密螺旋体属（Treponema）的高丰度富集，提示可能存在底泥厌氧和外源有机污染风险。④冗余分析（RDA）表明，4月样点与水温（WT）、叶绿素a（chl-a）和总磷（TP）的矢量方向较接近，10月部分样点与总氮（TN）、pH值、氟离子（F-）、电导率（EC）、总溶解性固体（TDS）和盐度（SAL）的矢量方向较一致。【结论】7月较高水温与氨氮水平有利于太阳山湿地水体细菌多样性和群落均衡，而春秋季低温、高氟及高盐度环境与细菌物种多样性下降相关，表明环境因子是驱动水体细菌群落季节性演替的关键因素。 |
| 关键词: 湿地 细菌群落 季节性演替 环境驱动因子 |
| DOI：10.13522/j.cnki.ggps.2026097 |
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| Seasonal variation in aquatic bacterial communities and their environmental drivers in an arid-region wetland |
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Zhang Xubin, Dong Junlin, Qiu Xiaocong, Yin Juan
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1. School of Chemistry and Chemical Engineering, Ningxia Normal University, Guyuan 756000, China;
2. Ningxia Guyuan No.4 Middle School, Guyuan 756000, China; 3. School of Civil and Hydraulic Engineering,
Ningxia University, Yinchuan 750021, China; 4. School of Life Sciences, Ningxia University, Yinchuan 750021, China
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| Abstract: |
| 【Objective】Aquatic ecosystems in arid regions are sensitive to environmental fluctuations. This study investigated seasonal variation in bacterial communities and the underlying environmental drivers in Taiyangshan Wetland, an arid-region wetland in Ningxia Province, China. 【Method】The study combined physicochemical analyses of water samples collected from the wetland, with high-throughput 16S rRNA gene sequencing to characterize seasonal changes in bacterial community structure and identify their environmental drivers.【Result】①The physicochemical properties of the aquatic environment in Taiyangshan Wetland showed significant seasonal variation. Ammonium concentrations were higher in July, whereas concentrations of total phosphorus and fluoride ions were higher in October; fluoride concentrations were also relatively high in April. ②Bacterial diversity in the water was significantly higher in summer than in spring and autumn (P＜0.05). The combined effects of temperature and nutrient availability were associated with greater bacterial richness in July, whereas low temperature and high-fluoride stress in April and October were environmental factors associated with the low bacterial community diversity. ③Proteobacteria, Bacteroidetes, and Actinobacteria were the dominant bacterial phyla throughout the three seasons. In addition, relatively high abundances of Desulfomicrobium and Treponema were detected at several sampling sites, potentially indicating anaerobic conditions in sediments and the influence of external organic inputs. ④Redundancy analysis showed that samples taken in April were more closely associated with water temperature, chlorophyll a and total phosphorus, whereas samples taken in October were more strongly associated with total nitrogen, pH, fluoride ions, electrical conductivity, total dissolved solids and water salinity.【Conclusion】High temperature and ammonium concentrations in July were associated with greater bacterial diversity and community evenness. In contrast, low temperature, high fluoride concentrations and water salinity in spring and autumn were associated with low bacterial diversity. These findings demonstrate that seasonal changes in physicochemical conditions, particularly temperature, nutrient availability, fluoride and water salinity, are important drivers of bacterial community variation in arid-region wetlands. |
| Key words: wetland bacterial community seasonal succession environmental driving factors |
