| 引用本文: | 薛红飞,冯 彪,唐 悦,等.AMF对复合盐胁迫下草莓根际微生物和酶活性的影响[J].灌溉排水学报,2021,(6):15-20. |
| XUE Hongfei,FENG Biao,TANG Yue,et al.AMF对复合盐胁迫下草莓根际微生物和酶活性的影响[J].灌溉排水学报,2021,(6):15-20. |
|
| 摘要: |
| 【目的】探索丛枝菌根真菌（AMF）对复合盐胁迫下草莓土壤微生态环境的作用效果。【方法】以‘甜查理’草莓（Fragaria × ananassa Duch.）为研究对象，以NaCl与Na2SO4质量浓度比为1∶1的混合盐模拟盐毒害，分别设置0、50 mmol/L和100 mmol/L共3种混合盐物质的量浓度，每种盐物质的量浓度设置接种与不接种AMF，共6个处理，研究了接种AMF对草莓根际土壤微生物数量和酶活性的影响。【结果】随着盐物质的量浓度增加，草莓植株的盐害指数增高；菌根侵染率随盐物质的量浓度的升高而降低；土壤微生物数量和脲酶、过氧化氢酶、蔗糖酶、淀粉酶活性随着盐物质的量浓度增加均降低。盐胁迫28 d后，3种盐物质的量浓度下接种AMF的草莓盐害指数均比不接种处理有明显降低，分别降低了10.91%、21.12%、23.93%。接种AMF提高了土壤脲酶、过氧化氢酶、蔗糖酶、淀粉酶活性以及微生物数量（P＜0.05）。复合盐胁迫下，微生物数量和酶活性间有显著或极显著的正相关关系，二者与盐害指数均呈显著的负相关关系（P＜0.05）。【结论】接种AMF改善了盐分胁迫下土壤微生态环境，有助于提高草莓对盐碱环境的适应性，缓解复合盐胁迫对草莓生长的伤害。 |
| 关键词: 盐胁迫；丛枝菌根；草莓；微生物；酶活性 |
| DOI：10.13522/j.cnki.ggps.2021030 |
| 分类号: |
| 基金项目: |
|
| Inoculation with Arbuscular Mycorrhizal Fungi Promotes Microbial and Enzymatic Activity in the Rhizosphere of Strawberry under Salinity Stress |
|
XUE Hongfei, FENG Biao, TANG Yue, LI Shiheng, LI Zhiwei, DENG Yuan, FAN Li
|
|
1. Key Laboratory of Water Cycle and Related Land Surface Processes,Institute of Geographical Sciences and Natural Resources Research, CAS, Beijing 100101, China;2. College of Resources and Environment, University of Chinese Academy of Sciences, UCAS, Beijing 100049, China
|
| Abstract: |
| 【Background】The climate in Inner Mongolia of China is suitable for growing strawberry but the shallow root system of the crop makes it prone to soil salinity. How to alleviate soil salinity and improve the resistance of strawberry against soil salinity is hence essential to safeguarding strawberry production in both fields and greenhouses that endured secondary soil salination.【Objective】The objective of this paper is to study the feasibility of using arbuscular mycorrhizal fungi (AMF) inoculation to improve physical and biogeochemical conditions of the rhizosphere of strawberry to improve its resistance against salinity stress.【Method】We used the cultivar of ‘Charlie sweet’ as the model plant and inoculated its roots with AMF. They were then grown in soils with a salinity gradient created by adding 0 to 100 mmol/L of solution of NaCl and Na2SO4 mixed at 1:1 ratio. In each treatment, we measured the number of microbes and the activity of urease, catalase, sucrase and amylase in the rhizosphere. 【Result】With the salt concentration increasing, the salinity index of the crop increased, while the rate of mycorrhizal infection, the number of soil microorganisms and the enzymatic activity all decreased. Compared with control without inoculation, 28 days after instigating the salt stress inoculation reduced the salt damage index of the strawberry by 10.91% to 23.93%, depending on salt concentration, 28 days after instigating the salt stress. Inoculation also increased the activity of all enzymes and the number of microorganisms at significant level (P＜0.05). Microbial population and enzymatic activity were positively correlated with each other at significant level, and they were both negatively correlated with salinity injury index at significant level (P＜0.05).【Conclusion】Inoculating the strawberry roots with AMF improved microbial population and enzymatic activity in its rhizosphere, thereby improving its resistance against soil salinity and the yield. |
| Key words: salinity stress; AMF; strawberry; microbial numbers; enzymatic activity |
