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| DOI：10.13522/j.cnki.ggps.20180444 |
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| Response of Roots of Potato at Seedling Stage to Water Stress |
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WANG Xiaojiao, MENG Meilian, CAO Chunmei , LU Chunxing, XU Fei
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1.College of Agronomy, Inner Mongolia Agricultural University, Hohhot 010019, China; 2.Inner Mongolia Academy of Agricultural & Animal husbandry Sciences, Hohhot 010031, China
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| Abstract: |
| 【Objective】 Root development at early stage has consequence for seasonal plant growth, and the purpose of this paper is to study the response of roots of potato at seedling stage to water stress. 【Method】 The variety of KEXIN 1 was used in the experiment. We analyzed the gene expression at transcriptional level using high-throughout sequencing of Illumina HiSeq TM 4000 from the transcriptome to study the response of root to water stress.【Result】① Expression of 1488 genes was identified at moderate water stress, in which 578 were up-regulated and 910 were down-regulated. At sever water stress, expression of 5 241 genes were identified, among which 1 905 were up-regulated and 3336 genes were down-regulated, with 369 regulated neither way. The difference in gene expression was associated with protein kinase, hydrolase, oxidoreductase, aquaporin, transporter, transcription factors, among others. ②The glutathione and its metabolic pathway were involved in positive regulation to resist water stress during germination stage. ③The signal transduction of hormones ABA, ET and CKs were activated, yet the transduction of IAA, JA and SA was inhibited. The signal transduction pathways of ABA and ET were involved in improving the resistance of plant to water stress. ④Under moderate to severe water stress, 409 protein kinase genes from 7 families and 107 protein phosphatase genes from 12 families were found to have expressed, which might contribute to the resistance of plant to water stress. Serine/threonine phosphatase (PP2C, PP2A) was involved in regulating the ABA signal transduction. ⑤There were 488 transcription genes from 54 families, some of which were involved in resistance of the plant to water stress. 【Conclusion】Under water stress, genes in roots of potato seedling expressed differently. Some protein kinases, protein phosphatases, oxidoreductases, transcription and those involved in biological metabolic pathways and signal transduction pathways were involved in plant resistance to water stress. |
| Key words: potato; water stress; transcriptomes; different genes expression; response mechanism; root system |
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