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| DOI：10.13522/j.cnki.ggps.2019414 |
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| Structure of Bacteria Community in the Nanhai Lake at Frozen |
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YANG Wenhuan, WU Fanchen, SHEN Han, LI Weiping, ZHANG Sheng
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1.Institute of Water Conservancy and Civil Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China;2.The Colloge of Environment & Energy Resources, Inner Mongola University of Science & Technology, Baotou 014010, China
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| Abstract: |
| 【Objective】 Change in structure of microbial community could alert functions of an aquatic ecosystem, and this paper analyses the bacterial communities at Nanhai lake in Baotou when it was frozen.【Method】 Bacteria in samples taken from both water and sediments from 12 sites across the lake were sequenced using the high-throughput sequencing, and the data was then analysed using the correlation thermogram. 【Result】 Bacterial diversity varied between the sampling sites. At the phylum level, Proteobacteria, Chloroflexi, Cyanobacteria, Bacteroidetes and Actinobacteria were most abundant, with the number of Proteobacteria and Chloroflexi much higher than others in the sediment, while Cyanobacteria (29%) and Actinobacteria (33%) were more dominant than others in the water; it was also found that the abundance of bacteria was higher in areas popular to tourists. At genus level, Thiobacillus, norank-f-anaerolineaceae, unclassified-p-Chloroflexi were dominant, with Thiobacillus 21% higher in the sediment and norank-f-anaerolineaceae 24% higher in the water. The redundancy analysis for relationship between bacterial communities and environmental factors revealed that total nitrogen (TN), dissolved oxygen and temperature had the greatest influence on structure of the bacterial community in the water, while TN, NH4+-N and COD impacted the bacteria community structure in the sediment more. As such, controlling contents of these elements is critical to avoid outbreak of bloom in the lake.【Conclusion】 There has been an increase in eutrophication in Nanhai Lake in winter when the lake is frozen, and Thiobacillus found in the sediment across the lake plays an important role in biogeochemical cycles of sediments. Its control is hence critical to keep the lake from becoming black and odorous. |
| Key words: bacteria; community structure; high-throughput sequencing; Nanhai Lake; winter |
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