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| DOI：10.13522/j.cnki.ggps.2020077 |
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| Effects of Fertilization on Physical and Chemical Properties and Bacterial Community of Reclaimed Soil from Coal Mining Subsidence |
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DING Jianing, LIANG Libao*, FENG Pengyan, XU Jianmin
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College of Resources and Environment, Shanxi Agricultural University, Taigu 030801, China
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| Abstract: |
| 【Objective】Research the effects of fertilization on the physical and chemical properties and bacterial community structure of reclaimed soil after coal mining collapse, to provide reference for soil fertility cultivation.【Method】Taking the reclaimed soil of mining subsidence area of Jincheng, Shanxi Province as the object area, four different fertilization treatments were set up, namely respectively no fertilization（CK）, organic fertilizer（M）, inorganic fertilizer（W）, 50% organic + 50% inorganic fertilizer（WM）. Illumina Miseq high-throughput sequencing platform was used to study the effects of different fertilizers on bacterial community structure, and the correlation between the change of bacterial community structure and soil physical and chemical properties was analyzed. 【Result】Compared with CK, ①Treatment M and WM significantly（p<0.05）increased the aggregate ratio of 1~2 mm and decreased the aggregate ratio of 0.053~0.25 mm and <0.053 mm. ②Treatment M significantly increased soil organic matter, total nitrogen, total phosphorus, while treatment W and WM significantly increased available nutrients. ③Treatment M increased the activity of phosphatase and invertase, while treatment W increased the activity of urease. ④Treatment M and WM increased the abundance of Proteus significantly, while treatment M and W increased the abundance of actinomycetes significantly. ⑤The results of redundancy analysis showed that total phosphorus, organic matter, alkali-hydrolyzed nitrogen and volume mass were the main reasons for the differences of bacterial community abundance. The order of the influence on the bacterial community abundance of different fertilization treatments was: M>W>WM>CK.【Conclusion】Fertilization improved the physical and chemical properties of reclaimed soil in different degrees. Total phosphorus, organic matter, alkali-hydrolyzed nitrogen and volume mass were the significant factors affecting the structure of soil microbial community. |
| Key words: fertilization; reclaimed soil; soil physical and chemical properties; bacterial community; environmental factors |
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