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| DOI：10.13522/j.cnki.ggps.2020305 |
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| Response of Metabolic Group Change in Grape Pulp to Soil Moisture at Veraison Stage |
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ZHANG Kun, WANG Yu’an, HAO Yan
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1.College of Biological and Agricultural Engineering, Weifang University, Weifang 261061, China;2.Institute of Fruit and Floriculture Research, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China
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| Abstract: |
| 【Background】The delayed cultivation of grape is mainly concentrated in northwest and Northeast China, where the accumulated temperature can not meet the normal ripening of grape, the grape sales time could be delayed to December each year, and with high economic benefits. The maturity regulation is an important aspect of grape delayed cultivation. Previous studies maily focused on the regulation of light and temperature, but few on the regulation of water during grape ripening period, and water regulation is the most easily realized agronomic measure in actual production. 【Objective】 The purpose of this paper is to explore the effect of increasing soil moisture on the metabolism of ‘Red Globe’grape pulp under the delayed cultivation.【Method】This test took the ‘Red Globe’grape of delayed cultivation as the material, and about 20 days before veraison, the soil volume moisture content controlled by the automatic irrigation system were 0.15 m3/m3（low moisture）, 0.18 m3/m3（medium moisture）and 0.24 m3/m3 (high moisture), respectively, and maintained until the end of the experiment. The non-target metabonomics of pulp under different water treatments were analyzed and compared by LC-MS.【Result】With the decrease of soil water supply, the accumulation of soluble solids in grape fruits increased in the same period. The metabolomics showed that there were 27 different metabolites (POS) in pulp of low moisture treatment compared with high moisture treatment, among them, the fold change values (FC) of 11 metabolites such as tectoridin and levulinic acid were up-regulated, while 16 metabolites such as butyraldehyde were down regulated, which were the characteristics of high fruit maturity. There were 13 up-regulated and 21 down-regulated metabolites in medium moisture treatment compared with high moisture treatment, and the FC value of methanone and epsilon-viniferin (down-regulated) were relatively small. There were 8 same metabolic pathways in the enriched metabolic pathway for low moisture treatment and medium moisture treatment compared with high moisture treatment, and the main metabolites in the metabolic pathway was pyruvate.【Conclusion】The ripening process of grape fruit would be delayed significantly due to the high soil water content, including the decrease of soluble solids, and the decrease of metabolites related to pigment accumulation, aroma and cell wall structure and so on. Pyruvate might be the most active metabolite at veraison stage. |
| Key words: grape; delayed cultivation; veraison; water; metabolomic |
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