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| DOI：10.13522/j.cnki.ggps.2022182 |
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| Soaking Oat Seeds with Uniconazole to Improve Their Germination and Resistance of Seedlings to Drought Stress |
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HE Liheng, HAO Jie, SHI Minli, JIA Xiaoyun, SONG Xi’e
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1. College of Agriculture, Shanxi Agricultural University, Taigu 030801, China;
2. College of Grassland Sciences, Shanxi Agricultural University, Taigu 030801, China;
3. College of Life Sciences, Shanxi Agricultural University, Taigu 030801, China
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| Abstract: |
| 【Objective】Promoting technologies are often used to improve seed germination. In this paper, we study the efficacy of soaking oat seeds with uniconazole in improving their germination and resistance of the seedlings against drought stress. 【Method】We compare two oat varieties, 0117-119 (V1) and YY11-18 (V2). Seeds of each variety are soaked by uniconazole at concentrations 0 mg/L (U1), 15 mg/L (U2), 30 mg/L (U3) and 45 mg/L (U4), respectively. The germination pattern and the seedling traits in each treatment is measured. 【Result】The highest germination potential and germination rate of V1 are 52.0% and 72.67% (p<0.05), achieved under U1 and U2, respectively. The least germination potential and germination rate are V2 under U4, being 18% and 36%, respectively. V1 under U1 achieves the highest seedling shoot length, maximum root length and crown root dry weight, while V2 under U4 gives the least. The number of lateral roots, root dry weight and root-crown ratio of V1 under U2 are significantly higher than those in other treatments (p<0.05), being 6.30, 4.33 mg and 0.34, respectively. V1 under U2 gives the best SOD, POD, CAT, root vigor and proline contents, being 150.22 U/g, 34.83 U/g, 387.35 U/g, 239.43 μg/(g·h) and 298.88 μg/g, respectively, but the lowest malondialdehyde content at 7.28 nmol/g. The contents of chlorophyll a and b, as well as the total chlorophyll in V1 under U4 are significantly higher than those in other treatments (p<0.05), being 1.51 mg/g, 0.58 mg/g and 2.12 mg/g, respectively. V1 is more drought resistant than V2. Soaking the seeds by uniconazole at concentration of 15 mg/L is optimal for both varieties, and V1 under U2 works the best. 【Conclusion】Soaking oat seeds with uniconazole can effectively alleviate the damage caused by drought, thereby improving their germination rate. Our results can help design oat seed drill in semi-arid areas that are facing drought stress in northern China. |
| Key words: Oat; uniconazole; drought stress; seed germination; drought-resistant physiology |
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